TY - JOUR
T1 - Volumetric in vivo imaging of microvascular perfusion within the intact cochlea in mice using ultra-high sensitive optical microangiography
AU - Subhash, Hrebesh M.
AU - Davila, Viviana
AU - Sun, Hai
AU - Nguyen-Huynh, Anh T.
AU - Shi, Xiaorui
AU - Nuttall, Alfred L.
AU - Wang, Ruikang K.
N1 - Funding Information:
Manuscript received May 04, 2010; revised July 21, 2010; accepted August 26, 2010. Date of publication September 02, 2010; date of current version February 02, 2011. This work was supported in part by the National Institute of Deafness and Other Communication Disorders under Grant R01DC010201, Grant R01DC010399, Grant R01 DC000105, and Grant R03DC008888 and in part by the National Center for Research Resources under Award KL2RR024141. The content is solely the responsibility of the authors and does not necessarily represent the official views of grant giving bodies. Asterisk indicates corresponding author.
PY - 2011/2
Y1 - 2011/2
N2 - Studying the inner ear microvascular dynamics is extremely important to understand the cochlear function and to further advance the diagnosis, prevention, and treatment of many otologic disorders. However, there is currently no effective imaging tool available that is able to access the blood flow within the intact cochlea. In this paper, we report the use of an ultrahigh sensitive optical micro-angiography (UHS-OMAG) imaging system to image 3-D microvascular perfusion within the intact cochlea in living mice. The UHS-OMAG image system used in this study is based on spectral domain optical coherence tomography, which uses a broadband light source centered at 1300 nm with an imaging rate of 47\thinspace 000 A-scans/s, capable of acquiring high-resolution B scans at 300 frames/s. The technique is sensitive enough to image very slow blood flow velocities, such as those found in capillary networks. The 3-D imaging acquisition time for a whole cochlea is ∼ 4.1 s. We demonstrate that volumetric reconstruction of microvascular flow obtained by UHS-OMAG provides a comprehensive perfusion map of several regions of the cochlea, including the otic capsule, the stria vascularis of the apical and middle turns and the radiating arterioles that emanate from the modiolus.
AB - Studying the inner ear microvascular dynamics is extremely important to understand the cochlear function and to further advance the diagnosis, prevention, and treatment of many otologic disorders. However, there is currently no effective imaging tool available that is able to access the blood flow within the intact cochlea. In this paper, we report the use of an ultrahigh sensitive optical micro-angiography (UHS-OMAG) imaging system to image 3-D microvascular perfusion within the intact cochlea in living mice. The UHS-OMAG image system used in this study is based on spectral domain optical coherence tomography, which uses a broadband light source centered at 1300 nm with an imaging rate of 47\thinspace 000 A-scans/s, capable of acquiring high-resolution B scans at 300 frames/s. The technique is sensitive enough to image very slow blood flow velocities, such as those found in capillary networks. The 3-D imaging acquisition time for a whole cochlea is ∼ 4.1 s. We demonstrate that volumetric reconstruction of microvascular flow obtained by UHS-OMAG provides a comprehensive perfusion map of several regions of the cochlea, including the otic capsule, the stria vascularis of the apical and middle turns and the radiating arterioles that emanate from the modiolus.
KW - Biomedical optical imaging
KW - blood flow measurement
KW - optical coherence tomography
KW - optical interferometry
KW - optical microangiography
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U2 - 10.1109/TMI.2010.2072934
DO - 10.1109/TMI.2010.2072934
M3 - Article
C2 - 20813632
AN - SCOPUS:79551604693
SN - 0278-0062
VL - 30
SP - 224
EP - 230
JO - IEEE Transactions on Medical Imaging
JF - IEEE Transactions on Medical Imaging
IS - 2
M1 - 5560860
ER -