Volumetric in vivo imaging of intracochlear microstructures in mice by high-speed spectral domain optical coherence tomography

Hrebesh M. Subhash, Viviana Davila, Hai Sun, Anh Nguyen-Huynh, Alfred Nuttall, Ruikang K. Wang

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

There is considerable interest in developing new methods for in vivo imaging of the complex anatomy of the mammalian cochlea for clinical as well as fundamental studies. In this study, we explored, the feasibility of spectral domain optical coherence tomography (SD-OCT) for 3-D in vivo imaging of the cochlea in mice. The SD-OCT system employed in this study used a broadband light source centered at 1300 nm, and the imaging speed of the system was 47,000 A-scans per second using the InGaAs camera. The system was capable of providing fully processed, high-resolution B-scan images [512 (axial)×128 (lateral) pixels] at 280 frames per sec. The 3-D imaging acquisition time for a whole cochlea was ∼0.45 sec. The traditional SD-OCT structural imaging algorithm was used to reconstruct 3-D cochlear morphology. We demonstrated that SD-OCT can be successfully used for in vivo imaging of important morphological features within the mouse cochlea, such as the otic capsule and structures within, including Reissner's membrane, the basilar membrane, tectorial membrane, organ of Corti, and modiolus of the apical and middle turns.

Original languageEnglish (US)
Article number036024
JournalJournal of Biomedical Optics
Volume15
Issue number3
DOIs
StatePublished - May 2010

Fingerprint

cochlea
Optical tomography
mice
tomography
high speed
Imaging techniques
microstructure
Microstructure
membranes
Membranes
anatomy
capsules
organs
acquisition
light sources
pixels
cameras
broadband
Capsules
Light sources

Keywords

  • Anatomy
  • Cochlea
  • In vivo imaging
  • Optical coherence tomography
  • Sensorineural hearing loss

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Volumetric in vivo imaging of intracochlear microstructures in mice by high-speed spectral domain optical coherence tomography. / Subhash, Hrebesh M.; Davila, Viviana; Sun, Hai; Nguyen-Huynh, Anh; Nuttall, Alfred; Wang, Ruikang K.

In: Journal of Biomedical Optics, Vol. 15, No. 3, 036024, 05.2010.

Research output: Contribution to journalArticle

Subhash, Hrebesh M. ; Davila, Viviana ; Sun, Hai ; Nguyen-Huynh, Anh ; Nuttall, Alfred ; Wang, Ruikang K. / Volumetric in vivo imaging of intracochlear microstructures in mice by high-speed spectral domain optical coherence tomography. In: Journal of Biomedical Optics. 2010 ; Vol. 15, No. 3.
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