Vitrification of caprine secondary and early antral follicles as a perspective to preserve fertility function

Everton Pimentel Ferreira Lopes, Giovanna Quintino Rodrigues, Danielle Cristina Calado de Brito, Rebeca Magalhães Pedrosa Rocha, Anna Clara Accioly Ferreira, Naíza Arcângela Ribeiro de Sá, Renato Félix da Silva, Gabriel Las Heras de Alcântara, Benner Geraldo Alves, José Ricardo de Figueiredo, Mary Zelinski, Ana Paula Ribeiro Rodrigues

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The present study aimed to evaluate the structure, survival and development of isolated caprine (secondary-SEC and early antral-EANT) follicles, after vitrification in the presence of synthetic polymers and in vitro culture. Additionally, transzonal projections (TZPs) and p450 aromatase enzyme were evaluated. After isolation, SEC and EANT follicles were in vitro cultured for six days or vitrified. After one week, SEC and EANT follicles were warmed and also in vitro cultured for six days. Data revealed that the percentage of morphologically normal follicles was similar between fresh and vitrified follicles in both follicular categories and antrum formation rate was similar between fresh and vitrified SEC follicles. Fluorescence by calcein-AM did not show difference between fresh and vitrified (SEC and EANT) follicles, however, the trypan blue test showed low viability for vitrified follicles. The integrity of TZPs was not affected between fresh and vitrified SEC follicles, however, in vitrified EANT follicles, there were signs of TZPs loss. Regarding steroidogenic function, it was observed a positive staining for p450 aromatase enzyme in fresh and vitrified SEC and EANT follicles. It was concluded that SEC follicles seem to be more resistant to vitrification than EANT follicles, as shown by the trypan blue test and TZPs assay. Future studies may confirm this hypothesis, in order to consolidate the use of SEC and EANT follicles as an alternative to ovary cryopreservation.

Original languageEnglish (US)
Pages (from-to)371-378
Number of pages8
JournalReproductive Biology
Volume20
Issue number3
DOIs
StatePublished - Sep 2020
Externally publishedYes

Keywords

  • Cryopreservation
  • Endocrine function
  • Goat
  • Ovarian follicles
  • TZPs

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology
  • Developmental Biology

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