Vitamin D inhibits angiogenesis in transgenic murine retinoblastoma

M. T. Shokravi, D. M. Marcus, J. Alroy, K. Egan, M. A. Saornil, D. M. Albert

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

Purpose. Vitamin D compounds have been shown to inhibit tumor growth in a transgenic retinoblastoma murine model. The mechanism of action has not been defined clearly, although an antiangiogenic action has been proposed. Methods. Transgenic retinoblastoma mice received high (0.05 μg) and low (0.025 μg) doses of vitamin D3 by intraperitoneal injection 5 times per week for 5 weeks. Control animals were injected with mineral oil vehicle alone. At 5 months of age, the animals were killed and eyes were enucleated and processed for light microscopy. Paraffin-embedded sections were stained with an immunoperoxidase stain (GS-1) specific for mammalian vascular endothelium. Sections were graded by a single masked reviewer, and intraobserver reliability was assessed. Mean vessel counts were made for each group. Results. The high-dose group had the lowest mean vessel count (8.5), followed by the low-dose group (10.1). The control group had the highest mean vessel count (14.1). Vitamin D-treated animals (high- and low-dose groups combined) had significantly fewer vessels (P = 0.001) than untreated controls. Conclusions. These results support the hypothesis that inhibition of angiogenesis is a mechanism of action for vitamin D in the transgenic retinoblastoma mouse model.

Original languageEnglish (US)
Pages (from-to)83-87
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume36
Issue number1
StatePublished - Jan 1 1995

Keywords

  • angiogenesis
  • retinoblastoma
  • transgenic mice
  • vitamin D3 compounds

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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    Shokravi, M. T., Marcus, D. M., Alroy, J., Egan, K., Saornil, M. A., & Albert, D. M. (1995). Vitamin D inhibits angiogenesis in transgenic murine retinoblastoma. Investigative Ophthalmology and Visual Science, 36(1), 83-87.