Vitamin C supplementation ameliorates the adverse effects of nicotine on placental hemodynamics and histology in nonhuman primates

Jamie O. Lo, Matthias C. Schabel, Victoria H.J. Roberts, Terry K. Morgan, Juha P. Rasanen, Christopher D. Kroenke, Sophie R. Shoemaker, Eliot R. Spindel, Antonio E. Frias

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Objective We previously demonstrated that prenatal nicotine exposure decreases neonatal pulmonary function in nonhuman primates, and maternal vitamin C supplementation attenuates these deleterious effects. However, the effect of nicotine on placental perfusion and development is not fully understood. This study utilizes noninvasive imaging techniques and histological analysis in a nonhuman primate model to test the hypothesis that prenatal nicotine exposure adversely effects placental hemodynamics and development but is ameliorated by vitamin C. Study Design Time-mated macaques (n = 27) were divided into 4 treatment groups: control (n = 5), nicotine only (n = 4), vitamin C only (n = 9), and nicotine plus vitamin C (n = 9). Nicotine animals received 2 mg/kg per day of nicotine bitartrate (approximately 0.7 mg/kg per day free nicotine levels in pregnant human smokers) from days 26 to 160 (term, 168 days). Vitamin C groups received ascorbic acid at 50, 100, or 250 mg/kg per day with or without nicotine. All underwent placental dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) at 135-140 days and Doppler ultrasound at 155 days to measure uterine artery and umbilical vein velocimetry and diameter to calculate uterine artery volume blood flow and placental volume blood flow. Animals were delivered by cesarean delivery at 160 days. A novel DCE-MRI protocol was utilized to calculate placental perfusion from maternal spiral arteries. Placental tissue was processed for histopathology. Results Placental volume blood flow was significantly reduced in nicotine-only animals compared with controls and nicotine plus vitamin C groups (P =.03). Maternal placental blood flow was not different between experimental groups by DCE-MRI, ranging from 0.75 to 1.94 mL/mL per minute (P =.93). Placental histology showed increased numbers of villous cytotrophoblast cell islands (P <.05) and increased syncytiotrophoblast sprouting (P <.001) in nicotine-only animals, which was mitigated by vitamin C. Conclusion Prenatal nicotine exposure significantly decreased fetal blood supply via reduced placental volume blood flow, which corresponded with placental histological findings previously associated with cigarette smoking. Vitamin C supplementation mitigated the harmful effects of prenatal nicotine exposure on placental hemodynamics and development, suggesting that its use may limit some of the adverse effects associated with smoking during pregnancy.

Original languageEnglish (US)
Pages (from-to)370.e1-370.e8
JournalAmerican journal of obstetrics and gynecology
Volume212
Issue number3
DOIs
StatePublished - Mar 1 2015

Keywords

  • nicotine
  • nonhuman primate
  • noninvasive imaging
  • placental perfusion
  • vitamin C

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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