Visualizing Synaptic Transfer of Tumor Antigens among Dendritic Cells

Megan K. Ruhland, Edward W. Roberts, En Cai, Adriana M. Mujal, Kyle Marchuk, Casey Beppler, David Nam, Nina K. Serwas, Mikhail Binnewies, Matthew F. Krummel

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Generation of tumor-infiltrating lymphocytes begins when tumor antigens reach the lymph node (LN) to stimulate T cells, yet we know little of how tumor material is disseminated among the large variety of antigen-presenting dendritic cell (DC) subsets in the LN. Here, we demonstrate that tumor proteins are carried to the LN within discrete vesicles inside DCs and are then transferred among DC subsets. A synapse is formed between interacting DCs and vesicle transfer takes place in the absence of free exosomes. DCs -containing vesicles can uniquely activate T cells, whereas DCs lacking them do not. Understanding this restricted sharing of tumor identity provides substantial room for engineering better anti-tumor immunity.

Original languageEnglish (US)
Pages (from-to)786-799.e5
JournalCancer Cell
Volume37
Issue number6
DOIs
StatePublished - Jun 8 2020
Externally publishedYes

Keywords

  • CD8+ T cells
  • T cell priming
  • antigen presentation
  • antigen trafficking
  • dendritic cells
  • lymph node
  • tumor immunology

ASJC Scopus subject areas

  • Oncology
  • Cell Biology
  • Cancer Research

Fingerprint

Dive into the research topics of 'Visualizing Synaptic Transfer of Tumor Antigens among Dendritic Cells'. Together they form a unique fingerprint.

Cite this