Visualization of virus-infected brain regions using a GFP-illuminating flashlight enables accurate and rapid dissection for biochemical analysis

Xuan Li, Marina Wolf

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Engineered viral vectors tagged with a fluorescent protein such as enhanced green fluorescent protein (EGFP) have been widely used to study neuronal function after intracranial injection into specific brain regions. A rapid dissection of the virus-expressing region is required for certain biochemical analyses. To improve the accuracy of the rapid dissection, we developed a method that employs a Bluestar flashlight in combination with barrier filter glasses to visualize the expression of EGFP lentivirus that has been microinjected into the nucleus accumbens. Processing of dissected tissue for EGFP immunoblotting further validated the approach.

Original languageEnglish (US)
Pages (from-to)177-179
Number of pages3
JournalJournal of Neuroscience Methods
Volume201
Issue number1
DOIs
StatePublished - Sep 30 2011
Externally publishedYes

Fingerprint

Dissection
Viruses
Brain
Lentivirus
Nucleus Accumbens
Immunoblotting
Glass
Injections
enhanced green fluorescent protein
Proteins

Keywords

  • Bluestar flashlight
  • EGFP
  • Lentivirus
  • Tissue dissection
  • Viral vector

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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