Visualization and Quantification of Phagocytosis by Neutrophils

Gaelen Guzman, Fikadu G. Tafesse

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Phagocytosis by phagocytes such as neutrophils is a crucial part of the host innate immune response against invading pathogens. Phagocytosis is a complex process that initiates with the binding of the particles on the cell surface of the phagocytes through the interaction of pattern recognition receptors with ligands on the surface of the pathogens. During this process, phagocytes undergo extensive membrane reorganization and cytoskeleton rearrangement at their cell surface. To gain better insight about the molecular mechanisms of this dynamic cellular process, visualization and quantification in a high-throughput manner is essential. Here, we describe a microscope-based method to visualize and quantify phagocytic uptake of pathogens (such as bacteria and fungi) and model particulates that are larger than 0.5 μm (such as Zymosan A and IgG-coated beads).

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages141-148
Number of pages8
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2087
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • High-content imaging
  • High-throughput
  • Neutrophils
  • Phagocytes
  • Phagocytosis
  • Phenotypic analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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