Visible light photoinitiation of mesenchymal stem cell-laden bioresponsive hydrogels

C. S. Bahney, T. J. Lujan, C. W. Hsu, M. Bottlang, J. L. West, Brian Johnstone

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Biological activity can be added to synthetic scaffolds by incorporating functional peptide sequences that provide enzyme-mediated degradation sites, facilitate cellular adhesion or stimulate signaling pathways. Poly(ethylene glycol) diacrylate is a popular synthetic base for tissue engineering scaffolds because it creates a hydrophilic environment that can be chemically manipulated to add this biological functionality. Furthermore, the acrylate groups allow for encapsulation of cells using photopolymerization under physiological conditions. One complication with the addition of these peptides is that aromatic amino acids absorb light at 285nm and compete with the ultraviolet (UV)-sensitive photoinitiators such as IrgacureTM 2959 (I2959), the most commonly used initiator for cytocompatible photoencapsulation of cells into synthetic scaffolds. In this study we define non-toxic conditions for photoencapsulation of human mesenchymal stem cells (hMSC) in PEGDA scaffolds using a visible light photoinitiator system composed of eosin Y, triethanolamine and 1-vinyl-2-pyrrolidinone. This visible light photoinitiator produced hydrogel scaffolds with an increased viability of encapsulated hMSCs and a more tightly crosslinked network in one-third the time of UV polymerization with I2959.

Original languageEnglish (US)
Pages (from-to)43-55
Number of pages13
JournalEuropean Cells and Materials
Volume22
StatePublished - Dec 2011

Fingerprint

Hydrogels
Stem cells
Mesenchymal Stromal Cells
Scaffolds
Scaffolds (biology)
Light
Peptides
Tissue Scaffolds
Artificial Cells
Triethanolamine
Aromatic Amino Acids
Photopolymerization
Hydrogel
Tissue Engineering
Eosine Yellowish-(YS)
Bioactivity
Carboxylic acids
Encapsulation
Tissue engineering
Polymerization

Keywords

  • Bioresponsive hydrogels
  • Dynamic modulus
  • Eosin Y
  • Mesenchymal stem cells
  • Photoencapsulation
  • Photopolymerization
  • Tissue engineering
  • Visible light photoinitiator

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials

Cite this

Bahney, C. S., Lujan, T. J., Hsu, C. W., Bottlang, M., West, J. L., & Johnstone, B. (2011). Visible light photoinitiation of mesenchymal stem cell-laden bioresponsive hydrogels. European Cells and Materials, 22, 43-55.

Visible light photoinitiation of mesenchymal stem cell-laden bioresponsive hydrogels. / Bahney, C. S.; Lujan, T. J.; Hsu, C. W.; Bottlang, M.; West, J. L.; Johnstone, Brian.

In: European Cells and Materials, Vol. 22, 12.2011, p. 43-55.

Research output: Contribution to journalArticle

Bahney, CS, Lujan, TJ, Hsu, CW, Bottlang, M, West, JL & Johnstone, B 2011, 'Visible light photoinitiation of mesenchymal stem cell-laden bioresponsive hydrogels', European Cells and Materials, vol. 22, pp. 43-55.
Bahney, C. S. ; Lujan, T. J. ; Hsu, C. W. ; Bottlang, M. ; West, J. L. ; Johnstone, Brian. / Visible light photoinitiation of mesenchymal stem cell-laden bioresponsive hydrogels. In: European Cells and Materials. 2011 ; Vol. 22. pp. 43-55.
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