@article{410134c214b24cffb4401556229894a6,
title = "Virus activated filopodia promote human papillomavirus type 31 uptake from the extracellular matrix",
abstract = "Human papillomaviruses (HPVs), etiological agents of epithelial tumors and cancers, initiate infection of basal human keratinocytes (HKs) facilitated by wounding. Virions bind to HKs and their secreted extracellular matrix (ECM), but molecular roles for wounding or ECM binding during infection are unclear. Herein we demonstrate that HPV31 activates signals promoting cytoskeletal rearrangements and virion transport required for internalization and infection. Activation of tyrosine and PI3 kinases precedes induction of filopodia whereon virions are transported toward the cell body. Coupled with the loss of ECM-bound virions this supports a model whereby virus activated filopodial transport contributes to an increased and protracted virion uptake into susceptible cells.",
keywords = "Cytoskeleton, Extracellular matrix, Filopodia, Keratinocyte, Papillomavirus, Signaling",
author = "Smith, {Jessica L.} and Lidke, {Diane S.} and Ozbun, {Michelle A.}",
note = "Funding Information: We are grateful to N. Fusenig (DKFZ, Heidelberg, DE) for HaCaT cells, Tom Jovin and Donna Arndt-Jovin for A431-erbB1-GFP cells (Max Planck Institute (Goettingen, DE), T. Kanda (National Institute of Infectious Diseases, Tokyo, JP) for the HPV31-L1/L2 expression plasmid, and N. Christensen (Penn State) for the H31.A6 monoclonal antibody. This work was supported by NIH Grant CA085747 (MAO), American Cancer Society IRG #192 (DSL), and NIH Training Grant T32 AI07538 (JLS). Anastacia Maldonado provided superb technical assistance. Fluorescent images in this paper were generated in the University of New Mexico Cancer Center Fluorescence Microscopy Facility, supported as detailed on the webpage: http://hsc.unm.edu/crtc/microscopy/Facility.html . We apologize to any investigators whose original articles could not be referenced due to space limitations. ",
year = "2008",
month = nov,
day = "10",
doi = "10.1016/j.virol.2008.08.040",
language = "English (US)",
volume = "381",
pages = "16--21",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",
}