Virtual histology of transgenic mouse embryos for high-throughput phenotyping.

John T. Johnson, Mark S. Hansen, Isabel Wu, Lindsey J. Healy, Christopher R. Johnson, Greg M. Jones, Mario R. Capecchi, Charles Keller

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

A bold new effort to disrupt every gene in the mouse genome necessitates systematic, interdisciplinary approaches to analyzing patterning defects in the mouse embryo. We present a novel, rapid, and inexpensive method for obtaining high-resolution virtual histology for phenotypic assessment of mouse embryos. Using osmium tetroxide to differentially stain tissues followed by volumetric X-ray computed tomography to image whole embryos, isometric resolutions of 27 mum or 8 mum were achieved with scan times of 2 h or 12 h, respectively, using mid-gestation E9.5-E12.5 embryos. The datasets generated by this method are immediately amenable to state-of-the-art computational methods of organ patterning analysis. This technique to assess embryo anatomy represents a significant improvement in resolution, time, and expense for the quantitative, three-dimensional analysis of developmental patterning defects attributed to genetically engineered mutations and chemically induced embryotoxicity.

Original languageEnglish (US)
JournalPLoS Genetics
Volume2
Issue number4
DOIs
StatePublished - Apr 2006
Externally publishedYes

Fingerprint

histology
Transgenic Mice
embryo
Histology
embryo (animal)
Embryonic Structures
genetically modified organisms
phenotype
mice
Chrysanthemum morifolium
defect
embryotoxicity
Osmium Tetroxide
osmium
interdisciplinary approach
X Ray Computed Tomography
computed tomography
methodology
pregnancy
anatomy

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Johnson, J. T., Hansen, M. S., Wu, I., Healy, L. J., Johnson, C. R., Jones, G. M., ... Keller, C. (2006). Virtual histology of transgenic mouse embryos for high-throughput phenotyping. PLoS Genetics, 2(4). https://doi.org/10.1371/journal.pgen.0020061

Virtual histology of transgenic mouse embryos for high-throughput phenotyping. / Johnson, John T.; Hansen, Mark S.; Wu, Isabel; Healy, Lindsey J.; Johnson, Christopher R.; Jones, Greg M.; Capecchi, Mario R.; Keller, Charles.

In: PLoS Genetics, Vol. 2, No. 4, 04.2006.

Research output: Contribution to journalArticle

Johnson, JT, Hansen, MS, Wu, I, Healy, LJ, Johnson, CR, Jones, GM, Capecchi, MR & Keller, C 2006, 'Virtual histology of transgenic mouse embryos for high-throughput phenotyping.', PLoS Genetics, vol. 2, no. 4. https://doi.org/10.1371/journal.pgen.0020061
Johnson, John T. ; Hansen, Mark S. ; Wu, Isabel ; Healy, Lindsey J. ; Johnson, Christopher R. ; Jones, Greg M. ; Capecchi, Mario R. ; Keller, Charles. / Virtual histology of transgenic mouse embryos for high-throughput phenotyping. In: PLoS Genetics. 2006 ; Vol. 2, No. 4.
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