Virion tails of Beet yellows virus

Coordinated assembly by three structural proteins

Dina Alzhanova, Alexey I. Prokhnevsky, Valera V. Peremyslov, Valerian V. Dolja

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Filamentous virions of Beet yellows virus contain a long body formed by a major capsid protein and a short tail that is assembled by a minor capsid protein (CPm), an Hsp70-homolog (Hsp70h), a 64-kDa protein (p64), and a 20-kDa protein (p20). Using mutation analysis and newly developed in planta assays, here we investigate the genetic requirements for the tail assembly. We show that the inactivation of CPm dramatically reduces incorporation of both Hsp70h and p64. Furthermore, inactivation of Hsp70h prevents incorporation of p64 into virions and vice versa. Hsp70h and p64 are each required for efficient incorporation of CPm. We also show that the tails possessing normal relative amounts of CPm, Hsp70h, and p64 can be formed in the absence of the major capsid protein and p20. Similar to the tails isolated from the wild-type virions, these mutant tails encapsidate the ∼ 700 nt-long, 5′-terminal segments of the viral RNA. Taken together, our results imply that CPm, Hsp70h and p64 act cooperatively to encapsidate a defined region of the closterovirus genome.

Original languageEnglish (US)
Pages (from-to)220-226
Number of pages7
JournalVirology
Volume359
Issue number1
DOIs
StatePublished - Mar 1 2007

Fingerprint

Closterovirus
Virus Assembly
Capsid Proteins
Virion
Proteins
Viral RNA
Genome
Mutation

Keywords

  • Closterovirus
  • Helical virion
  • Hsp70
  • Virus assembly

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Alzhanova, D., Prokhnevsky, A. I., Peremyslov, V. V., & Dolja, V. V. (2007). Virion tails of Beet yellows virus: Coordinated assembly by three structural proteins. Virology, 359(1), 220-226. https://doi.org/10.1016/j.virol.2006.09.007

Virion tails of Beet yellows virus : Coordinated assembly by three structural proteins. / Alzhanova, Dina; Prokhnevsky, Alexey I.; Peremyslov, Valera V.; Dolja, Valerian V.

In: Virology, Vol. 359, No. 1, 01.03.2007, p. 220-226.

Research output: Contribution to journalArticle

Alzhanova, D, Prokhnevsky, AI, Peremyslov, VV & Dolja, VV 2007, 'Virion tails of Beet yellows virus: Coordinated assembly by three structural proteins', Virology, vol. 359, no. 1, pp. 220-226. https://doi.org/10.1016/j.virol.2006.09.007
Alzhanova, Dina ; Prokhnevsky, Alexey I. ; Peremyslov, Valera V. ; Dolja, Valerian V. / Virion tails of Beet yellows virus : Coordinated assembly by three structural proteins. In: Virology. 2007 ; Vol. 359, No. 1. pp. 220-226.
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