Viral interleukin-6 encoded by rhesus macaque rhadinovirus is associated with lymphoproliferative disorder (LPD)

B. U. Orzechowska, M. Manoharan, J. Sprague, Ryan Estep, Michael Axthelm, Scott Wong

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Rhesus macaques (RM) co-infected with simian immunodeficiency virus (SIV) and rhesus macaque rhadinovirus (RRV) develop abnormal cellular proliferations characterized as extra-nodal lymphoma and retroperitoneal fibromatosis (RF). RRV encodes a viral interleukin-6 (vIL-6), much like Kaposi's sarcoma-associated herpesvirus, and involvement of the viral cytokine was examined in proliferative lesions. Methods: Formalin fixed tissue from RM co-infected with SIV and RRV were analyzed for RRV genomes by in situ hybridization and RRV vIL-6 expression by immunofluorescence analysis. Results: In situ hybridization analysis indicated that RRV is present in both types of lesions. Immunofluorescence analysis of different lymphomas and RF revealed positive staining for vIL-6. Similarly to KS, RF lesion is positive for vimentin, CD117 (c-kit), and smooth muscle actin (SMA) and contains T cell, B cell and monocytes/macrophage infiltrates. Conclusions: Our data support the idea that vIL-6 may be critical to the development and progression of lymphoproliferative disorder in RRV/SIV-infected RM.

Original languageEnglish (US)
Pages (from-to)2-7
Number of pages6
JournalJournal of Medical Primatology
Volume38
Issue numberSUPPL. 1
DOIs
StatePublished - Oct 2009

Fingerprint

Rhadinovirus
lymphatic diseases
Lymphoproliferative Disorders
Macaca mulatta
interleukin-6
Interleukin-6
Simian immunodeficiency virus
Simian Immunodeficiency Virus
Fibroma
lymphoma
in situ hybridization
fluorescent antibody technique
In Situ Hybridization
Fluorescent Antibody Technique
Lymphoma
Human herpesvirus 8
Human Herpesvirus 8
vimentin
Vimentin
formalin

Keywords

  • Multi-centric Castleman's disease
  • Neoplasia
  • RRV and SIV

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Viral interleukin-6 encoded by rhesus macaque rhadinovirus is associated with lymphoproliferative disorder (LPD). / Orzechowska, B. U.; Manoharan, M.; Sprague, J.; Estep, Ryan; Axthelm, Michael; Wong, Scott.

In: Journal of Medical Primatology, Vol. 38, No. SUPPL. 1, 10.2009, p. 2-7.

Research output: Contribution to journalArticle

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AU - Orzechowska, B. U.

AU - Manoharan, M.

AU - Sprague, J.

AU - Estep, Ryan

AU - Axthelm, Michael

AU - Wong, Scott

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N2 - Background: Rhesus macaques (RM) co-infected with simian immunodeficiency virus (SIV) and rhesus macaque rhadinovirus (RRV) develop abnormal cellular proliferations characterized as extra-nodal lymphoma and retroperitoneal fibromatosis (RF). RRV encodes a viral interleukin-6 (vIL-6), much like Kaposi's sarcoma-associated herpesvirus, and involvement of the viral cytokine was examined in proliferative lesions. Methods: Formalin fixed tissue from RM co-infected with SIV and RRV were analyzed for RRV genomes by in situ hybridization and RRV vIL-6 expression by immunofluorescence analysis. Results: In situ hybridization analysis indicated that RRV is present in both types of lesions. Immunofluorescence analysis of different lymphomas and RF revealed positive staining for vIL-6. Similarly to KS, RF lesion is positive for vimentin, CD117 (c-kit), and smooth muscle actin (SMA) and contains T cell, B cell and monocytes/macrophage infiltrates. Conclusions: Our data support the idea that vIL-6 may be critical to the development and progression of lymphoproliferative disorder in RRV/SIV-infected RM.

AB - Background: Rhesus macaques (RM) co-infected with simian immunodeficiency virus (SIV) and rhesus macaque rhadinovirus (RRV) develop abnormal cellular proliferations characterized as extra-nodal lymphoma and retroperitoneal fibromatosis (RF). RRV encodes a viral interleukin-6 (vIL-6), much like Kaposi's sarcoma-associated herpesvirus, and involvement of the viral cytokine was examined in proliferative lesions. Methods: Formalin fixed tissue from RM co-infected with SIV and RRV were analyzed for RRV genomes by in situ hybridization and RRV vIL-6 expression by immunofluorescence analysis. Results: In situ hybridization analysis indicated that RRV is present in both types of lesions. Immunofluorescence analysis of different lymphomas and RF revealed positive staining for vIL-6. Similarly to KS, RF lesion is positive for vimentin, CD117 (c-kit), and smooth muscle actin (SMA) and contains T cell, B cell and monocytes/macrophage infiltrates. Conclusions: Our data support the idea that vIL-6 may be critical to the development and progression of lymphoproliferative disorder in RRV/SIV-infected RM.

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KW - Neoplasia

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