Vicinal thiols are involved in inositol 1,2,3,5,6-pentakisphosphate 5-phosphatase activity from fetal calf thymus

Uday Bandyopadhyay, Thorsten Kaiser, Marco T. Rudolf, Carsten Schultz, Andreas H. Guse, Georg W. Mayr

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Inositol 1,2,3,5,6-pentakisphosphate (Ins(1,2,3,5,6)P5) 5-phosphatase present in fetal calf thymus has been partially purified. This enzyme was inhibited dose-dependently by different thiol modifiers like N-ethylmaleimide (NEM), p-chloromercuribenzene sulfonate (PCMBS), diamide, and phenylarsine oxide (PAO). The inhibition by PCMBS and diamide was protected by preincubation with dithiothreitol (DTT) and the phosphatase substrate, Ins(1,2,3,5,6)P5. Diamide, a compound that specifically modifies vicinal thiol groups, also blocked the 5-phosphatase dose-dependently. Specificity of this blockade was proven by using dimercaptopropanol (DMP), a compound known to protect vicinal thiol groups. DMP prevented the enzyme from inhibition by diamide. These data suggest that vicinal thiols are involved in Ins(1,2,3,5,6)P5 5-phosphatase activity.

Original languageEnglish (US)
Pages (from-to)146-149
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume240
Issue number1
DOIs
StatePublished - Nov 7 1997
Externally publishedYes

Fingerprint

Diamide
Thymus
Fetal Movement
Sulfhydryl Compounds
Thymus Gland
Phosphoric Monoester Hydrolases
Ethylmaleimide
Dithiothreitol
Inositol
Enzymes
inositol 1,2,3,5,6-pentakisphosphate 5-phosphatase
Substrates

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Vicinal thiols are involved in inositol 1,2,3,5,6-pentakisphosphate 5-phosphatase activity from fetal calf thymus. / Bandyopadhyay, Uday; Kaiser, Thorsten; Rudolf, Marco T.; Schultz, Carsten; Guse, Andreas H.; Mayr, Georg W.

In: Biochemical and Biophysical Research Communications, Vol. 240, No. 1, 07.11.1997, p. 146-149.

Research output: Contribution to journalArticle

Bandyopadhyay, Uday ; Kaiser, Thorsten ; Rudolf, Marco T. ; Schultz, Carsten ; Guse, Andreas H. ; Mayr, Georg W. / Vicinal thiols are involved in inositol 1,2,3,5,6-pentakisphosphate 5-phosphatase activity from fetal calf thymus. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 240, No. 1. pp. 146-149.
@article{9ea706b41409423fa79183a5ef66dcca,
title = "Vicinal thiols are involved in inositol 1,2,3,5,6-pentakisphosphate 5-phosphatase activity from fetal calf thymus",
abstract = "Inositol 1,2,3,5,6-pentakisphosphate (Ins(1,2,3,5,6)P5) 5-phosphatase present in fetal calf thymus has been partially purified. This enzyme was inhibited dose-dependently by different thiol modifiers like N-ethylmaleimide (NEM), p-chloromercuribenzene sulfonate (PCMBS), diamide, and phenylarsine oxide (PAO). The inhibition by PCMBS and diamide was protected by preincubation with dithiothreitol (DTT) and the phosphatase substrate, Ins(1,2,3,5,6)P5. Diamide, a compound that specifically modifies vicinal thiol groups, also blocked the 5-phosphatase dose-dependently. Specificity of this blockade was proven by using dimercaptopropanol (DMP), a compound known to protect vicinal thiol groups. DMP prevented the enzyme from inhibition by diamide. These data suggest that vicinal thiols are involved in Ins(1,2,3,5,6)P5 5-phosphatase activity.",
author = "Uday Bandyopadhyay and Thorsten Kaiser and Rudolf, {Marco T.} and Carsten Schultz and Guse, {Andreas H.} and Mayr, {Georg W.}",
year = "1997",
month = "11",
day = "7",
doi = "10.1006/bbrc.1997.7629",
language = "English (US)",
volume = "240",
pages = "146--149",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Vicinal thiols are involved in inositol 1,2,3,5,6-pentakisphosphate 5-phosphatase activity from fetal calf thymus

AU - Bandyopadhyay, Uday

AU - Kaiser, Thorsten

AU - Rudolf, Marco T.

AU - Schultz, Carsten

AU - Guse, Andreas H.

AU - Mayr, Georg W.

PY - 1997/11/7

Y1 - 1997/11/7

N2 - Inositol 1,2,3,5,6-pentakisphosphate (Ins(1,2,3,5,6)P5) 5-phosphatase present in fetal calf thymus has been partially purified. This enzyme was inhibited dose-dependently by different thiol modifiers like N-ethylmaleimide (NEM), p-chloromercuribenzene sulfonate (PCMBS), diamide, and phenylarsine oxide (PAO). The inhibition by PCMBS and diamide was protected by preincubation with dithiothreitol (DTT) and the phosphatase substrate, Ins(1,2,3,5,6)P5. Diamide, a compound that specifically modifies vicinal thiol groups, also blocked the 5-phosphatase dose-dependently. Specificity of this blockade was proven by using dimercaptopropanol (DMP), a compound known to protect vicinal thiol groups. DMP prevented the enzyme from inhibition by diamide. These data suggest that vicinal thiols are involved in Ins(1,2,3,5,6)P5 5-phosphatase activity.

AB - Inositol 1,2,3,5,6-pentakisphosphate (Ins(1,2,3,5,6)P5) 5-phosphatase present in fetal calf thymus has been partially purified. This enzyme was inhibited dose-dependently by different thiol modifiers like N-ethylmaleimide (NEM), p-chloromercuribenzene sulfonate (PCMBS), diamide, and phenylarsine oxide (PAO). The inhibition by PCMBS and diamide was protected by preincubation with dithiothreitol (DTT) and the phosphatase substrate, Ins(1,2,3,5,6)P5. Diamide, a compound that specifically modifies vicinal thiol groups, also blocked the 5-phosphatase dose-dependently. Specificity of this blockade was proven by using dimercaptopropanol (DMP), a compound known to protect vicinal thiol groups. DMP prevented the enzyme from inhibition by diamide. These data suggest that vicinal thiols are involved in Ins(1,2,3,5,6)P5 5-phosphatase activity.

UR - http://www.scopus.com/inward/record.url?scp=0031558764&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031558764&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1997.7629

DO - 10.1006/bbrc.1997.7629

M3 - Article

C2 - 9367900

AN - SCOPUS:0031558764

VL - 240

SP - 146

EP - 149

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -