Vesicular dopamine release elicits an inhibitory postsynaptic current in midbrain dopamine neurons

Michael J. Beckstead, David Grandy, Kevin Wickman, John Williams

Research output: Contribution to journalArticle

200 Citations (Scopus)

Abstract

Synchronous activation of dopamine neurons, for instance upon presentation of an unexpected rewarding stimulus, results in the release of dopamine from both terminals in projection areas and somatodendritic sites within the ventral midbrain. This report describes an inhibitory postsynaptic current (IPSC) that was elicited by dopamine in slices from mouse midbrain. The IPSC was tetrodotoxin sensitive, calcium dependent, and blocked by a D2 receptor antagonist. Inhibition of monoamine transporters prolonged the IPSC, indicating that the time course of dopamine neurotransmission is tightly regulated by reuptake. Changing the stimulus intensity altered the amplitude but not the time course of the IPSC, whose onset was faster than could be reproduced with iontophoresis. The results indicate a rapid rise in dopamine concentration at the D2 receptors, suggesting that dopamine that is released by a train of action potentials acts in a localized area rather than in a manner consistent with volume transmission.

Original languageEnglish (US)
Pages (from-to)939-946
Number of pages8
JournalNeuron
Volume42
Issue number6
DOIs
StatePublished - Jun 24 2004

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Inhibitory Postsynaptic Potentials
Dopaminergic Neurons
Mesencephalon
Dopamine
Iontophoresis
Dopamine D2 Receptors
Tetrodotoxin
Synaptic Transmission
Action Potentials
Calcium

ASJC Scopus subject areas

  • Neuroscience(all)

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Vesicular dopamine release elicits an inhibitory postsynaptic current in midbrain dopamine neurons. / Beckstead, Michael J.; Grandy, David; Wickman, Kevin; Williams, John.

In: Neuron, Vol. 42, No. 6, 24.06.2004, p. 939-946.

Research output: Contribution to journalArticle

Beckstead, Michael J. ; Grandy, David ; Wickman, Kevin ; Williams, John. / Vesicular dopamine release elicits an inhibitory postsynaptic current in midbrain dopamine neurons. In: Neuron. 2004 ; Vol. 42, No. 6. pp. 939-946.
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