Vertical transmission of diverse microbes in the tropical sponge Corticium sp.

Koty H. Sharp, Boreth Eam, D. John Faulkner, Margo Haygood

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Sponges are host to extremely diverse bacterial communities, some of which appear to be spatiotemporally stable, though how these consistent associations are assembled and maintained from one sponge generation to the next is not well understood. Here we report that a diverse group of microbes, including both bacteria and archaea, is consistently present in aggregates within embryos of the tropical sponge Corticium sp. The major taxonomic groups represented in bacterial 16S rRNA sequences amplified from the embryos are similar to those previously described in a variety of marine sponges. Three selected bacterial taxa, representing proteobacteria, actinobacteria, and a clade including recently described sponge-associated bacteria, were tested and found to be present in all adult samples tested over a 3-year period and in the embryos throughout development. Specific probes were used in fluorescence in situ hybridization to localize cells of the three types in the embryos and mesohyl. This study confirms the vertical transmission of multiple, phylogenetically diverse microorganisms in a marine sponge, and our findings lay the foundation for future work on exploring vertical transmission of specific, yet diverse, microbial assemblages in marine sponges.

Original languageEnglish (US)
Pages (from-to)622-629
Number of pages8
JournalApplied and Environmental Microbiology
Volume73
Issue number2
DOIs
StatePublished - Jan 2007

Fingerprint

Corticium
vertical transmission
Porifera
sponge
microorganisms
embryo
embryo (animal)
Embryonic Structures
Bacteria
Proteobacteria
bacterium
Actinobacteria
Archaea
bacteria
cell aggregates
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
bacterial communities
Embryonic Development
fluorescence

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Vertical transmission of diverse microbes in the tropical sponge Corticium sp. / Sharp, Koty H.; Eam, Boreth; John Faulkner, D.; Haygood, Margo.

In: Applied and Environmental Microbiology, Vol. 73, No. 2, 01.2007, p. 622-629.

Research output: Contribution to journalArticle

Sharp, Koty H. ; Eam, Boreth ; John Faulkner, D. ; Haygood, Margo. / Vertical transmission of diverse microbes in the tropical sponge Corticium sp. In: Applied and Environmental Microbiology. 2007 ; Vol. 73, No. 2. pp. 622-629.
@article{3752a822061f4329b7a758eb36917671,
title = "Vertical transmission of diverse microbes in the tropical sponge Corticium sp.",
abstract = "Sponges are host to extremely diverse bacterial communities, some of which appear to be spatiotemporally stable, though how these consistent associations are assembled and maintained from one sponge generation to the next is not well understood. Here we report that a diverse group of microbes, including both bacteria and archaea, is consistently present in aggregates within embryos of the tropical sponge Corticium sp. The major taxonomic groups represented in bacterial 16S rRNA sequences amplified from the embryos are similar to those previously described in a variety of marine sponges. Three selected bacterial taxa, representing proteobacteria, actinobacteria, and a clade including recently described sponge-associated bacteria, were tested and found to be present in all adult samples tested over a 3-year period and in the embryos throughout development. Specific probes were used in fluorescence in situ hybridization to localize cells of the three types in the embryos and mesohyl. This study confirms the vertical transmission of multiple, phylogenetically diverse microorganisms in a marine sponge, and our findings lay the foundation for future work on exploring vertical transmission of specific, yet diverse, microbial assemblages in marine sponges.",
author = "Sharp, {Koty H.} and Boreth Eam and {John Faulkner}, D. and Margo Haygood",
year = "2007",
month = "1",
doi = "10.1128/AEM.01493-06",
language = "English (US)",
volume = "73",
pages = "622--629",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Vertical transmission of diverse microbes in the tropical sponge Corticium sp.

AU - Sharp, Koty H.

AU - Eam, Boreth

AU - John Faulkner, D.

AU - Haygood, Margo

PY - 2007/1

Y1 - 2007/1

N2 - Sponges are host to extremely diverse bacterial communities, some of which appear to be spatiotemporally stable, though how these consistent associations are assembled and maintained from one sponge generation to the next is not well understood. Here we report that a diverse group of microbes, including both bacteria and archaea, is consistently present in aggregates within embryos of the tropical sponge Corticium sp. The major taxonomic groups represented in bacterial 16S rRNA sequences amplified from the embryos are similar to those previously described in a variety of marine sponges. Three selected bacterial taxa, representing proteobacteria, actinobacteria, and a clade including recently described sponge-associated bacteria, were tested and found to be present in all adult samples tested over a 3-year period and in the embryos throughout development. Specific probes were used in fluorescence in situ hybridization to localize cells of the three types in the embryos and mesohyl. This study confirms the vertical transmission of multiple, phylogenetically diverse microorganisms in a marine sponge, and our findings lay the foundation for future work on exploring vertical transmission of specific, yet diverse, microbial assemblages in marine sponges.

AB - Sponges are host to extremely diverse bacterial communities, some of which appear to be spatiotemporally stable, though how these consistent associations are assembled and maintained from one sponge generation to the next is not well understood. Here we report that a diverse group of microbes, including both bacteria and archaea, is consistently present in aggregates within embryos of the tropical sponge Corticium sp. The major taxonomic groups represented in bacterial 16S rRNA sequences amplified from the embryos are similar to those previously described in a variety of marine sponges. Three selected bacterial taxa, representing proteobacteria, actinobacteria, and a clade including recently described sponge-associated bacteria, were tested and found to be present in all adult samples tested over a 3-year period and in the embryos throughout development. Specific probes were used in fluorescence in situ hybridization to localize cells of the three types in the embryos and mesohyl. This study confirms the vertical transmission of multiple, phylogenetically diverse microorganisms in a marine sponge, and our findings lay the foundation for future work on exploring vertical transmission of specific, yet diverse, microbial assemblages in marine sponges.

UR - http://www.scopus.com/inward/record.url?scp=33846426845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846426845&partnerID=8YFLogxK

U2 - 10.1128/AEM.01493-06

DO - 10.1128/AEM.01493-06

M3 - Article

C2 - 17122394

AN - SCOPUS:33846426845

VL - 73

SP - 622

EP - 629

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 2

ER -