Vav proteins regulate the plasma cell program and secretory Ig production

Linda M. Stephenson, Ana V. Miletic, Tracie Kloeppel, Shana Kusin, Wojciech Swat

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Plasma cell (PC) development is initiated following B cell activation and controlled by a B lymphocyte-induced maturation protein (Blimp)-1-dependent program involving the concerted action of several proplasma transcriptional regulators. However, the factors that control Blimp-1 expression remain largely unknown. In this context, mice deficient for all three of the Vav Family of proteins (Vavnull) develop substantial B cell populations, including marginal zone B cells, yet have a virtual absence of serum Igs, indicating that Vav may be specifically required in PC development and Ig production. We show in this study that mature marginal zone B cells from Vavnull mice proliferate following stimulation with TLR ligands but exhibit severe defects in PC differentiation and Ig secretion. Under conditions inducing PC differentiation, Vavnull B cells fail to efficiently induce Blimp-1, X box-binding protein-1, J chain, or secretory Ig μ transcripts but express IFN-regulatory factor-4 at levels similar to wild-type cells. These data indicate a previously unknown role for Vav as an upstream regulator of Blimp-1.

Original languageEnglish (US)
Pages (from-to)8620-8625
Number of pages6
JournalJournal of Immunology
Volume177
Issue number12
StatePublished - Dec 15 2006
Externally publishedYes

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Plasma Cells
B-Lymphocytes
Proteins
Cell Differentiation
Ligands
Serum
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Stephenson, L. M., Miletic, A. V., Kloeppel, T., Kusin, S., & Swat, W. (2006). Vav proteins regulate the plasma cell program and secretory Ig production. Journal of Immunology, 177(12), 8620-8625.

Vav proteins regulate the plasma cell program and secretory Ig production. / Stephenson, Linda M.; Miletic, Ana V.; Kloeppel, Tracie; Kusin, Shana; Swat, Wojciech.

In: Journal of Immunology, Vol. 177, No. 12, 15.12.2006, p. 8620-8625.

Research output: Contribution to journalArticle

Stephenson, LM, Miletic, AV, Kloeppel, T, Kusin, S & Swat, W 2006, 'Vav proteins regulate the plasma cell program and secretory Ig production', Journal of Immunology, vol. 177, no. 12, pp. 8620-8625.
Stephenson LM, Miletic AV, Kloeppel T, Kusin S, Swat W. Vav proteins regulate the plasma cell program and secretory Ig production. Journal of Immunology. 2006 Dec 15;177(12):8620-8625.
Stephenson, Linda M. ; Miletic, Ana V. ; Kloeppel, Tracie ; Kusin, Shana ; Swat, Wojciech. / Vav proteins regulate the plasma cell program and secretory Ig production. In: Journal of Immunology. 2006 ; Vol. 177, No. 12. pp. 8620-8625.
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