Vav binds to several SH2/SH3 containing proteins in activated lymphocytes

Francisco Ramos-Morales, Brian Druker, Siegmund Fischer

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

In T lymphocytes, several proteins are rapidly phosphorylated on tyrosine after stimulation. In this study we examine the ability of tyrosine phosphorylated proteins from Jurkat T cells stimulated by CD2 or T cell receptor-CD3 to interact with the src homology 2 or src homology 3 domains from eight different proteins involved in signal transduction in lymphocytes: Vav, Shc, Nck, phosphatidylinositol-3-kinase, phospholipase C-γ 1, Ras-GTPase activating protein, c-Crk and Grb2. Our data show that different SH2 domains have distinct patterns of binding to phosphotyrosine containing proteins. We show that Vav, a protein expressed only in hematopoietic cells that may have guanine nucleotide releasing factor activity, is able to interact with certain SH2-containing proteins depending on its tyrosine phosphorylation and with Grb2 in a manner independent of phosphorylation on tyrosine. Coimmunoprecipitation experiments support the idea of a trimolecular complex Shc-Grb2-Vav in vivo. These data suggest a central role played by Vav and provide insight in the complexity and specificity of protein-protein interactions in the signaling events in lymphocytes.

Original languageEnglish (US)
Pages (from-to)1917-1923
Number of pages7
JournalOncogene
Volume9
Issue number7
StatePublished - Jul 1994
Externally publishedYes

Fingerprint

Lymphocytes
Tyrosine
Proteins
src Homology Domains
ras GTPase-Activating Proteins
Phosphorylation
Phosphatidylinositol 3-Kinase
T-Lymphocytes
Guanine Nucleotide Exchange Factors
Phosphotyrosine
Jurkat Cells
Type C Phospholipases
T-Cell Antigen Receptor
Signal Transduction

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Ramos-Morales, F., Druker, B., & Fischer, S. (1994). Vav binds to several SH2/SH3 containing proteins in activated lymphocytes. Oncogene, 9(7), 1917-1923.

Vav binds to several SH2/SH3 containing proteins in activated lymphocytes. / Ramos-Morales, Francisco; Druker, Brian; Fischer, Siegmund.

In: Oncogene, Vol. 9, No. 7, 07.1994, p. 1917-1923.

Research output: Contribution to journalArticle

Ramos-Morales, F, Druker, B & Fischer, S 1994, 'Vav binds to several SH2/SH3 containing proteins in activated lymphocytes', Oncogene, vol. 9, no. 7, pp. 1917-1923.
Ramos-Morales, Francisco ; Druker, Brian ; Fischer, Siegmund. / Vav binds to several SH2/SH3 containing proteins in activated lymphocytes. In: Oncogene. 1994 ; Vol. 9, No. 7. pp. 1917-1923.
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