Vault ribonucleoprotein particles from rat and bullfrog contain a related small RNA that is transcribed by RNA polymerase III

Valerie A. Kickhoefer, Robert Searles, Nancy L. Kedersha, Mitchell E. Garber, Deborah L. Johnson, Leonard H. Rome

    Research output: Contribution to journalArticle

    84 Citations (Scopus)

    Abstract

    Vaults are large cytoplasmic ribonucleoprotein particles with a sedimentation value of about 150 S. These particles contain a unique small RNA (vault RNA (vRNA)). We have determined the sequence of the RNA associated with vaults purified from both rat and bull-frog. The rat vRNA is 141 bases in length, whereas the bullfrog vRNA is present as two highly related species of 89 and 94 bases. Despite the differences in length the predicted secondary structures of the three vRNAs are clearly related. All of the vRNAs contain sequences related to the internal promoter elements necessary for transcription by RNA polymerase III. The gene for the rat vRNA was isolated and sequenced from a rat genomic library, and its transcription by RNA polymerase III was verified using an in vitro transcription assay. The rat vRNA gene was efficiently transcribed in vitro, producing a single transcript of about 140 bases. Unlike most RNA polymerase III genes, the rat vRNA is present as a single copy gene and has a distinct tissue-specific expression pattern.

    Original languageEnglish (US)
    Pages (from-to)7868-7873
    Number of pages6
    JournalJournal of Biological Chemistry
    Volume268
    Issue number11
    StatePublished - Apr 15 1993

    Fingerprint

    Vault Ribonucleoprotein Particles
    RNA Polymerase III
    Rana catesbeiana
    Rats
    RNA
    Transcription
    Genes
    Ribonucleoproteins
    Genomic Library
    Anura
    Sedimentation
    Assays

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Kickhoefer, V. A., Searles, R., Kedersha, N. L., Garber, M. E., Johnson, D. L., & Rome, L. H. (1993). Vault ribonucleoprotein particles from rat and bullfrog contain a related small RNA that is transcribed by RNA polymerase III. Journal of Biological Chemistry, 268(11), 7868-7873.

    Vault ribonucleoprotein particles from rat and bullfrog contain a related small RNA that is transcribed by RNA polymerase III. / Kickhoefer, Valerie A.; Searles, Robert; Kedersha, Nancy L.; Garber, Mitchell E.; Johnson, Deborah L.; Rome, Leonard H.

    In: Journal of Biological Chemistry, Vol. 268, No. 11, 15.04.1993, p. 7868-7873.

    Research output: Contribution to journalArticle

    Kickhoefer, Valerie A. ; Searles, Robert ; Kedersha, Nancy L. ; Garber, Mitchell E. ; Johnson, Deborah L. ; Rome, Leonard H. / Vault ribonucleoprotein particles from rat and bullfrog contain a related small RNA that is transcribed by RNA polymerase III. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 11. pp. 7868-7873.
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    AU - Searles, Robert

    AU - Kedersha, Nancy L.

    AU - Garber, Mitchell E.

    AU - Johnson, Deborah L.

    AU - Rome, Leonard H.

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