Vasodilator effects on canine basilar artery induced by intracisternal interleukin-1β

Koji Osuka, Yoshio Suzuki, Yasuo Watanabe, Aclan Dogan, Masakazu Takayasu, Masato Shibuya, Jun Yoshida

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The effect of interleukin-1β (IL-1β) on a cerebral artery was investigated in anesthetized dogs. Intracisternal administration of IL-1β (0.03 and 0.3 μg) dilated the canine basilar artery in a dose-dependent manner, without affecting systemic blood pressure or heart rate. The increase in diameter induced by 0.3 μg of IL-1β was 28.4% ± 13.4% of control at 2 hours and was inhibited by 30 μg of the IL-1β receptor antagonist, zinc protoporphyrin (4.5% ± 13.5%, P <0.05). Interleukin-1β did not affect the concentration of nitric oxide metabolites in CSF. However, there was an increase in the concentration of eicosanoids in CSF, and the elevation of 6- keto-PGF1(α) paralleled the vasodilation. Pretreatment with 30 μg of the selective inducible cyclooxygenase (COX-2) inhibitor NS-398 also inhibited the IL-1β-induced vasodilation significantly (5.9% ± 9.4% at 2 hours, P <0.01). Western blot analysis revealed the expression of s 68-kD COX-2-like protein in basilar artery extracts. These findings suggest that the IL-1β- induced vasodilator effect is linked to the prostaglandin cascade, predominantly to prostaglandin I2, by induction of COX-2, but not to the stimulation of nitric oxide metabolism.

Original languageEnglish (US)
Pages (from-to)1337-1345
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume17
Issue number12
StatePublished - Dec 1997
Externally publishedYes

Fingerprint

Basilar Artery
Vasodilator Agents
Interleukin-1
Canidae
Cyclooxygenase 2 Inhibitors
Vasodilation
Nitric Oxide
Cerebral Arteries
Interleukin-1 Receptors
Eicosanoids
Epoprostenol
Prostaglandins
Heart Rate
Western Blotting
Dogs
Blood Pressure
Proteins

Keywords

  • Canine cerebral artery
  • Eicosanoids
  • Inducible cyclooxygenase (COX- 2)
  • Nitric oxide
  • Vasodilation

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Osuka, K., Suzuki, Y., Watanabe, Y., Dogan, A., Takayasu, M., Shibuya, M., & Yoshida, J. (1997). Vasodilator effects on canine basilar artery induced by intracisternal interleukin-1β. Journal of Cerebral Blood Flow and Metabolism, 17(12), 1337-1345.

Vasodilator effects on canine basilar artery induced by intracisternal interleukin-1β. / Osuka, Koji; Suzuki, Yoshio; Watanabe, Yasuo; Dogan, Aclan; Takayasu, Masakazu; Shibuya, Masato; Yoshida, Jun.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 17, No. 12, 12.1997, p. 1337-1345.

Research output: Contribution to journalArticle

Osuka, K, Suzuki, Y, Watanabe, Y, Dogan, A, Takayasu, M, Shibuya, M & Yoshida, J 1997, 'Vasodilator effects on canine basilar artery induced by intracisternal interleukin-1β', Journal of Cerebral Blood Flow and Metabolism, vol. 17, no. 12, pp. 1337-1345.
Osuka, Koji ; Suzuki, Yoshio ; Watanabe, Yasuo ; Dogan, Aclan ; Takayasu, Masakazu ; Shibuya, Masato ; Yoshida, Jun. / Vasodilator effects on canine basilar artery induced by intracisternal interleukin-1β. In: Journal of Cerebral Blood Flow and Metabolism. 1997 ; Vol. 17, No. 12. pp. 1337-1345.
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