Vasoactive intestinal polypeptide gene expression in the developing human gastrointestinal tract

Paul Facer, Anne E. Bishop, Gonzalo Moscoso, Giorgio Terenghi, Yu F. Liu, Richard Goodman, Stephen Legon, Julia M. Polak

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Expression of vasoactive intestinal polypeptide has been shown, by immunocytochemistry and biochemical assay, to follow the craniocaudal neural colonization of the mammalian gut. The aim of this study was to use in situ hybridization to see if it could provide more information on vasoactive intestinal polypeptide gene expression in the developing human gut. Immunocytochemistry of vasoactive intestinal polypeptide and, to visualize the total innervation, protein gene product 9.5 was also applied. By 8 weeks of gestation, protein gene product 9.5-immunoreactive neurons had colonized the gut lengthwise (17% of intestinal muscle area) but not transversely. Vasoactive intestinal polypeptide immunoreactivity was first detected at 9 weeks of gestation in a few nerve fibers of the upper gut, the origin of which could not be determined. Vasoactive intestinal polypeptide-immunoreactive ganglion cells were not seen until 18 weeks of gestation, whereas in situ hybridization showed messenger RNA in ganglion cells of the upper gut at 9 weeks. An adultlike pattern of peptide gene products (e.g., 2.5% and 3.1% of intestinal mucosal or muscle area, respectively) was detected by 20 weeks' gestation. The finding that the vasoactive intestinal polypeptide gene is expressed first in the upper human gut is consistent with craniocaudal neuronal colonization and maturation.

Original languageEnglish (US)
Pages (from-to)47-55
Number of pages9
JournalGastroenterology
Volume102
Issue number1
StatePublished - 1992

Fingerprint

Vasoactive Intestinal Peptide
Gastrointestinal Tract
Gene Expression
Pregnancy
Ganglia
In Situ Hybridization
Immunohistochemistry
Muscles
Nerve Fibers
Genes
Proteins
Neurons
Messenger RNA
Peptides

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Facer, P., Bishop, A. E., Moscoso, G., Terenghi, G., Liu, Y. F., Goodman, R., ... Polak, J. M. (1992). Vasoactive intestinal polypeptide gene expression in the developing human gastrointestinal tract. Gastroenterology, 102(1), 47-55.

Vasoactive intestinal polypeptide gene expression in the developing human gastrointestinal tract. / Facer, Paul; Bishop, Anne E.; Moscoso, Gonzalo; Terenghi, Giorgio; Liu, Yu F.; Goodman, Richard; Legon, Stephen; Polak, Julia M.

In: Gastroenterology, Vol. 102, No. 1, 1992, p. 47-55.

Research output: Contribution to journalArticle

Facer, P, Bishop, AE, Moscoso, G, Terenghi, G, Liu, YF, Goodman, R, Legon, S & Polak, JM 1992, 'Vasoactive intestinal polypeptide gene expression in the developing human gastrointestinal tract', Gastroenterology, vol. 102, no. 1, pp. 47-55.
Facer, Paul ; Bishop, Anne E. ; Moscoso, Gonzalo ; Terenghi, Giorgio ; Liu, Yu F. ; Goodman, Richard ; Legon, Stephen ; Polak, Julia M. / Vasoactive intestinal polypeptide gene expression in the developing human gastrointestinal tract. In: Gastroenterology. 1992 ; Vol. 102, No. 1. pp. 47-55.
@article{6c6de644bc5c43efa62bfd2dd2ceb27f,
title = "Vasoactive intestinal polypeptide gene expression in the developing human gastrointestinal tract",
abstract = "Expression of vasoactive intestinal polypeptide has been shown, by immunocytochemistry and biochemical assay, to follow the craniocaudal neural colonization of the mammalian gut. The aim of this study was to use in situ hybridization to see if it could provide more information on vasoactive intestinal polypeptide gene expression in the developing human gut. Immunocytochemistry of vasoactive intestinal polypeptide and, to visualize the total innervation, protein gene product 9.5 was also applied. By 8 weeks of gestation, protein gene product 9.5-immunoreactive neurons had colonized the gut lengthwise (17{\%} of intestinal muscle area) but not transversely. Vasoactive intestinal polypeptide immunoreactivity was first detected at 9 weeks of gestation in a few nerve fibers of the upper gut, the origin of which could not be determined. Vasoactive intestinal polypeptide-immunoreactive ganglion cells were not seen until 18 weeks of gestation, whereas in situ hybridization showed messenger RNA in ganglion cells of the upper gut at 9 weeks. An adultlike pattern of peptide gene products (e.g., 2.5{\%} and 3.1{\%} of intestinal mucosal or muscle area, respectively) was detected by 20 weeks' gestation. The finding that the vasoactive intestinal polypeptide gene is expressed first in the upper human gut is consistent with craniocaudal neuronal colonization and maturation.",
author = "Paul Facer and Bishop, {Anne E.} and Gonzalo Moscoso and Giorgio Terenghi and Liu, {Yu F.} and Richard Goodman and Stephen Legon and Polak, {Julia M.}",
year = "1992",
language = "English (US)",
volume = "102",
pages = "47--55",
journal = "Gastroenterology",
issn = "0016-5085",
publisher = "W.B. Saunders Ltd",
number = "1",

}

TY - JOUR

T1 - Vasoactive intestinal polypeptide gene expression in the developing human gastrointestinal tract

AU - Facer, Paul

AU - Bishop, Anne E.

AU - Moscoso, Gonzalo

AU - Terenghi, Giorgio

AU - Liu, Yu F.

AU - Goodman, Richard

AU - Legon, Stephen

AU - Polak, Julia M.

PY - 1992

Y1 - 1992

N2 - Expression of vasoactive intestinal polypeptide has been shown, by immunocytochemistry and biochemical assay, to follow the craniocaudal neural colonization of the mammalian gut. The aim of this study was to use in situ hybridization to see if it could provide more information on vasoactive intestinal polypeptide gene expression in the developing human gut. Immunocytochemistry of vasoactive intestinal polypeptide and, to visualize the total innervation, protein gene product 9.5 was also applied. By 8 weeks of gestation, protein gene product 9.5-immunoreactive neurons had colonized the gut lengthwise (17% of intestinal muscle area) but not transversely. Vasoactive intestinal polypeptide immunoreactivity was first detected at 9 weeks of gestation in a few nerve fibers of the upper gut, the origin of which could not be determined. Vasoactive intestinal polypeptide-immunoreactive ganglion cells were not seen until 18 weeks of gestation, whereas in situ hybridization showed messenger RNA in ganglion cells of the upper gut at 9 weeks. An adultlike pattern of peptide gene products (e.g., 2.5% and 3.1% of intestinal mucosal or muscle area, respectively) was detected by 20 weeks' gestation. The finding that the vasoactive intestinal polypeptide gene is expressed first in the upper human gut is consistent with craniocaudal neuronal colonization and maturation.

AB - Expression of vasoactive intestinal polypeptide has been shown, by immunocytochemistry and biochemical assay, to follow the craniocaudal neural colonization of the mammalian gut. The aim of this study was to use in situ hybridization to see if it could provide more information on vasoactive intestinal polypeptide gene expression in the developing human gut. Immunocytochemistry of vasoactive intestinal polypeptide and, to visualize the total innervation, protein gene product 9.5 was also applied. By 8 weeks of gestation, protein gene product 9.5-immunoreactive neurons had colonized the gut lengthwise (17% of intestinal muscle area) but not transversely. Vasoactive intestinal polypeptide immunoreactivity was first detected at 9 weeks of gestation in a few nerve fibers of the upper gut, the origin of which could not be determined. Vasoactive intestinal polypeptide-immunoreactive ganglion cells were not seen until 18 weeks of gestation, whereas in situ hybridization showed messenger RNA in ganglion cells of the upper gut at 9 weeks. An adultlike pattern of peptide gene products (e.g., 2.5% and 3.1% of intestinal mucosal or muscle area, respectively) was detected by 20 weeks' gestation. The finding that the vasoactive intestinal polypeptide gene is expressed first in the upper human gut is consistent with craniocaudal neuronal colonization and maturation.

UR - http://www.scopus.com/inward/record.url?scp=0026599357&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026599357&partnerID=8YFLogxK

M3 - Article

C2 - 1530785

AN - SCOPUS:0026599357

VL - 102

SP - 47

EP - 55

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 1

ER -