Vasoactive intestinal peptide activates Ca2+ -dependent K+ channels through a cAMP pathway in mouse lacrimal cells

James D. Lechleiter, Darlene A. Dartt, Paul Brehm

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The action of vasoactive intestinal peptide (VIP) on Ca2+ -dependent K+ currents, in dissociated mouse lacrimal cells, was investigated using patch clamp techniques. In whole cell recordings, VIP (10-100 pM) increased the magnitude of the Ca2+-dependent K+ current. In single channel recordings, VIP increased the fraction of time the large chary bdotoxin-sensitive Ca2+-activated K+ channel spent in the open state. The activity of this channel was also increased by adding forskolin or 8-bromo cAMP to the bath. Additionally, application of either cAMP or catalytic subunit of cAMP-dependent protein kinase directly to the cytoplasmic surface of excised inside out patches reversibly lengthened the time Ca2+-activated K+ channels spent in the open state. These data suggest that VIP stimulates Ca2+-activated K+ channels by a cAMR-dependent pathway in mouse lacrimal acinar cells.

Original languageEnglish (US)
Pages (from-to)227-235
Number of pages9
JournalNeuron
Volume1
Issue number3
DOIs
StatePublished - 1988
Externally publishedYes

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Vasoactive Intestinal Peptide
Tears
Calcium-Activated Potassium Channels
Patch-Clamp Techniques
8-Bromo Cyclic Adenosine Monophosphate
Acinar Cells
Colforsin
Cyclic AMP-Dependent Protein Kinases
Baths
Catalytic Domain

ASJC Scopus subject areas

  • Neuroscience(all)

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Vasoactive intestinal peptide activates Ca2+ -dependent K+ channels through a cAMP pathway in mouse lacrimal cells. / Lechleiter, James D.; Dartt, Darlene A.; Brehm, Paul.

In: Neuron, Vol. 1, No. 3, 1988, p. 227-235.

Research output: Contribution to journalArticle

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