Vascular recruitment in skeletal muscle during exercise and hyperinsulinemia assessed by contrast ultrasound

Dana Dawson, Michelle A. Vincent, Eugene J. Barrett, Sanjiv Kaul, Andrew Clark, Howard Leong-Poi, Jonathan Lindner

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

The purpose of this study was to non-invasively quantify the effects of insulin on capillary blood volume (capBV) and RBC velocity (VRBC) in skeletal muscle in vivo with the use of contrast-enhanced ultrasound. We performed contrast ultrasound of the rat hindlimb adductor muscles at baseline and after 2-h infusions of either insulin (3 or 40 mU·kg-1.min-1) or saline. Saline-treated animals were also studied during contractile exercise. VRBC and capBV were calculated from the relation between pulsing interval and video intensity. Femoral artery blood flow, measured by a flow probe, increased with both contractile exercise and insulin. Contractile exercise increased capBV more than twofold and VRBC fivefold. Insulin also increased capBV more than twofold in a dose-dependent fashion but did not significantly alter VRBC. Saline infusion did not significantly alter capBV, VRBC, or femoral artery blood flow. We conclude that physiological changes in skeletal muscle capillary perfusion can be assessed in vivo with the use of contrast-enhanced ultrasound. Exercise increases both VRBC and capBV, whereas hyperinsulinemia selectively increases only capBV, which may enhance skeletal muscle glucose uptake.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume282
Issue number3 45-3
StatePublished - 2002
Externally publishedYes

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Hyperinsulinism
Blood Vessels
Blood Volume
Muscle
Skeletal Muscle
Blood
Ultrasonics
Insulin
Femoral Artery
Hindlimb
Perfusion
Rats
Animals
Glucose
Muscles

Keywords

  • Blood volume
  • Capillaries
  • Insulin

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Vascular recruitment in skeletal muscle during exercise and hyperinsulinemia assessed by contrast ultrasound. / Dawson, Dana; Vincent, Michelle A.; Barrett, Eugene J.; Kaul, Sanjiv; Clark, Andrew; Leong-Poi, Howard; Lindner, Jonathan.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 282, No. 3 45-3, 2002.

Research output: Contribution to journalArticle

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