Abstract
The purpose of this study was to non-invasively quantify the effects of insulin on capillary blood volume (capBV) and RBC velocity (VRBC) in skeletal muscle in vivo with the use of contrast-enhanced ultrasound. We performed contrast ultrasound of the rat hindlimb adductor muscles at baseline and after 2-h infusions of either insulin (3 or 40 mU·kg-1.min-1) or saline. Saline-treated animals were also studied during contractile exercise. VRBC and capBV were calculated from the relation between pulsing interval and video intensity. Femoral artery blood flow, measured by a flow probe, increased with both contractile exercise and insulin. Contractile exercise increased capBV more than twofold and VRBC fivefold. Insulin also increased capBV more than twofold in a dose-dependent fashion but did not significantly alter VRBC. Saline infusion did not significantly alter capBV, VRBC, or femoral artery blood flow. We conclude that physiological changes in skeletal muscle capillary perfusion can be assessed in vivo with the use of contrast-enhanced ultrasound. Exercise increases both VRBC and capBV, whereas hyperinsulinemia selectively increases only capBV, which may enhance skeletal muscle glucose uptake.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Endocrinology and Metabolism |
| Volume | 282 |
| Issue number | 3 45-3 |
| State | Published - 2002 |
| Externally published | Yes |
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Keywords
- Blood volume
- Capillaries
- Insulin
ASJC Scopus subject areas
- Physiology
- Endocrinology
- Biochemistry
Cite this
Vascular recruitment in skeletal muscle during exercise and hyperinsulinemia assessed by contrast ultrasound. / Dawson, Dana; Vincent, Michelle A.; Barrett, Eugene J.; Kaul, Sanjiv; Clark, Andrew; Leong-Poi, Howard; Lindner, Jonathan.
In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 282, No. 3 45-3, 2002.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vascular recruitment in skeletal muscle during exercise and hyperinsulinemia assessed by contrast ultrasound
AU - Dawson, Dana
AU - Vincent, Michelle A.
AU - Barrett, Eugene J.
AU - Kaul, Sanjiv
AU - Clark, Andrew
AU - Leong-Poi, Howard
AU - Lindner, Jonathan
PY - 2002
Y1 - 2002
N2 - The purpose of this study was to non-invasively quantify the effects of insulin on capillary blood volume (capBV) and RBC velocity (VRBC) in skeletal muscle in vivo with the use of contrast-enhanced ultrasound. We performed contrast ultrasound of the rat hindlimb adductor muscles at baseline and after 2-h infusions of either insulin (3 or 40 mU·kg-1.min-1) or saline. Saline-treated animals were also studied during contractile exercise. VRBC and capBV were calculated from the relation between pulsing interval and video intensity. Femoral artery blood flow, measured by a flow probe, increased with both contractile exercise and insulin. Contractile exercise increased capBV more than twofold and VRBC fivefold. Insulin also increased capBV more than twofold in a dose-dependent fashion but did not significantly alter VRBC. Saline infusion did not significantly alter capBV, VRBC, or femoral artery blood flow. We conclude that physiological changes in skeletal muscle capillary perfusion can be assessed in vivo with the use of contrast-enhanced ultrasound. Exercise increases both VRBC and capBV, whereas hyperinsulinemia selectively increases only capBV, which may enhance skeletal muscle glucose uptake.
AB - The purpose of this study was to non-invasively quantify the effects of insulin on capillary blood volume (capBV) and RBC velocity (VRBC) in skeletal muscle in vivo with the use of contrast-enhanced ultrasound. We performed contrast ultrasound of the rat hindlimb adductor muscles at baseline and after 2-h infusions of either insulin (3 or 40 mU·kg-1.min-1) or saline. Saline-treated animals were also studied during contractile exercise. VRBC and capBV were calculated from the relation between pulsing interval and video intensity. Femoral artery blood flow, measured by a flow probe, increased with both contractile exercise and insulin. Contractile exercise increased capBV more than twofold and VRBC fivefold. Insulin also increased capBV more than twofold in a dose-dependent fashion but did not significantly alter VRBC. Saline infusion did not significantly alter capBV, VRBC, or femoral artery blood flow. We conclude that physiological changes in skeletal muscle capillary perfusion can be assessed in vivo with the use of contrast-enhanced ultrasound. Exercise increases both VRBC and capBV, whereas hyperinsulinemia selectively increases only capBV, which may enhance skeletal muscle glucose uptake.
KW - Blood volume
KW - Capillaries
KW - Insulin
UR - http://www.scopus.com/inward/record.url?scp=0036087465&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036087465&partnerID=8YFLogxK
M3 - Article
C2 - 11832377
AN - SCOPUS:0036087465
VL - 282
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 3 45-3
ER -