Vascular endothelial growth factor (VEGF) and angiopoietin regulation by gonadotrophin and steroids in macaque granulosa cells during the peri-ovulatory interval

T. M. Hazzard, T. A. Molskness, C. L. Chaffin, Richard Stouffer

    Research output: Contribution to journalArticle

    126 Citations (Scopus)

    Abstract

    The role of endothelial cell-specific growth factors in the vascularization of the primate peri-ovulatory follicle was examined. Experiments were designed firstly to detect expression of vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) in granulosa cells and secondly, to determine whether gonadotrophins and/or steroids regulate their expression during the peri-ovulatory interval. Granulosa cells and follicular fluid were collected from rhesus macaques undergoing ovarian stimulation before (0 h), 12, or 36 h after a bolus of ovulatory human chorionic gonadotrophin (HCG), with or without steroid ablation and progestin replacement. VEGF, Ang-1 and Ang-2 mRNA were all detected prior to the ovulatory stimulus. Whereas follicular fluid VEGF concentrations increased 6-fold (P <0.05) between 0 and 12 h, VEGF mRNA values were unchanged and were unaffected by steroid ablation. Ang-1 mRNA decreased from 0 to 12 h (P<0.05), followed by a 30-fold increase (P <0.05) at 36 h, while Ang-2 mRNA values were unchanged between 0, 12 and 36 h. Steroid ablation decreased (P <0.05) Ang-1 mRNA at 36 h, and Ang-2 mRNA at 12 h, while only Ang- 1 was restored by progestin replacement. These data suggest a dynamic expression of vascular-specific growth factors in a gonadotrophin-dependent, steroid-independent (VEGF) or steroid- dependent (Ang-1) manner in granulosa cells of peri-ovulatory follicles of primates.

    Original languageEnglish (US)
    Pages (from-to)1115-1121
    Number of pages7
    JournalMolecular Human Reproduction
    Volume5
    Issue number12
    StatePublished - Dec 1999

    Fingerprint

    Angiopoietins
    Angiopoietin-1
    Granulosa Cells
    Macaca
    Gonadotropins
    Angiopoietin-2
    Vascular Endothelial Growth Factor A
    Steroids
    Messenger RNA
    Follicular Fluid
    Progestins
    Primates
    Intercellular Signaling Peptides and Proteins
    Ovulation Induction
    Chorionic Gonadotropin
    Macaca mulatta
    Blood Vessels
    Endothelial Cells

    Keywords

    • Angiopoietin-1
    • Angiopoietin-2
    • Granulosa cells
    • VEGF

    ASJC Scopus subject areas

    • Obstetrics and Gynecology
    • Genetics
    • Developmental Biology
    • Embryology
    • Cell Biology

    Cite this

    Vascular endothelial growth factor (VEGF) and angiopoietin regulation by gonadotrophin and steroids in macaque granulosa cells during the peri-ovulatory interval. / Hazzard, T. M.; Molskness, T. A.; Chaffin, C. L.; Stouffer, Richard.

    In: Molecular Human Reproduction, Vol. 5, No. 12, 12.1999, p. 1115-1121.

    Research output: Contribution to journalArticle

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