Vascular endothelial cells promote acute plasticity in ependymoglial cells of the neuroendocrine brain

Sandrine De Seranno, Cecilia Estrella, Anne Loyens, Anda Cornea, Sergio R. Ojeda, Jean Claude Beauvillain, Vincent Prevot

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

Glial and endothelial cells interact throughout the brain to define specific functional domains. Whether endothelial cells convey signals to glia in the mature brain is unknown but is amenable to examination in circumventricular organs. Here we report that purified endothelial cells of one of these organs, the median eminence of the hypothalamus, induce acute actin cytoskeleton remodeling in isolated ependymoglial cells and show that this plasticity is mediated by nitric oxide (NO), a diffusible factor. We found that both soluble guanylyl cyclase and cyclooxygenase products are involved in this endothelial-mediated control of ependymoglia cytoarchitecture. We also demonstrate by electron microscopy that activation of endogenous NO release in the median eminence induces rapid structural changes, allowing a direct access of neurosecretory axons containing gonadotropin-releasing hormone (GnRH) (the neuropeptide controlling reproductive function) to the portal vasculature. Local in vivo inhibition of NO synthesis disrupts reproductive cycliciry, a process that requires a pulsatile, coordinated delivery of GnRH into the hypothalamic-adenohypophyseal portal system. Our results identify a previously unknown function for endothelial cells in inducing neuroglial plasticity and raise the intriguing possibility that endothelial cells throughout the brain may use a similar signaling mechanism to regulate glial-neuronal interactions.

Original languageEnglish (US)
Pages (from-to)10353-10363
Number of pages11
JournalJournal of Neuroscience
Volume24
Issue number46
DOIs
StatePublished - Nov 17 2004
Externally publishedYes

Keywords

  • Endothelial nitric oxide synthase
  • Glial cells
  • Hypothalamus
  • Neuroglial plasticity
  • Neurosecretion
  • Tanycytes

ASJC Scopus subject areas

  • Neuroscience(all)

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