Vascular endothelial cells promote acute plasticity in ependymoglial cells of the neuroendocrine brain

Sandrine De Seranno, Cecilia Estrella, Anne Loyens, Anda Cornea, Sergio Ojeda, Jean Claude Beauvillain, Vincent Prevot

    Research output: Contribution to journalArticle

    52 Citations (Scopus)

    Abstract

    Glial and endothelial cells interact throughout the brain to define specific functional domains. Whether endothelial cells convey signals to glia in the mature brain is unknown but is amenable to examination in circumventricular organs. Here we report that purified endothelial cells of one of these organs, the median eminence of the hypothalamus, induce acute actin cytoskeleton remodeling in isolated ependymoglial cells and show that this plasticity is mediated by nitric oxide (NO), a diffusible factor. We found that both soluble guanylyl cyclase and cyclooxygenase products are involved in this endothelial-mediated control of ependymoglia cytoarchitecture. We also demonstrate by electron microscopy that activation of endogenous NO release in the median eminence induces rapid structural changes, allowing a direct access of neurosecretory axons containing gonadotropin-releasing hormone (GnRH) (the neuropeptide controlling reproductive function) to the portal vasculature. Local in vivo inhibition of NO synthesis disrupts reproductive cycliciry, a process that requires a pulsatile, coordinated delivery of GnRH into the hypothalamic-adenohypophyseal portal system. Our results identify a previously unknown function for endothelial cells in inducing neuroglial plasticity and raise the intriguing possibility that endothelial cells throughout the brain may use a similar signaling mechanism to regulate glial-neuronal interactions.

    Original languageEnglish (US)
    Pages (from-to)10353-10363
    Number of pages11
    JournalJournal of Neuroscience
    Volume24
    Issue number46
    DOIs
    StatePublished - Nov 17 2004

    Fingerprint

    Ependymoglial Cells
    Endothelial Cells
    Neuroglia
    Brain
    Median Eminence
    Nitric Oxide
    Gonadotropin-Releasing Hormone
    Portal System
    Prostaglandin-Endoperoxide Synthases
    Neuropeptides
    Actin Cytoskeleton
    Hypothalamus
    Axons
    Electron Microscopy

    Keywords

    • Endothelial nitric oxide synthase
    • Glial cells
    • Hypothalamus
    • Neuroglial plasticity
    • Neurosecretion
    • Tanycytes

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Vascular endothelial cells promote acute plasticity in ependymoglial cells of the neuroendocrine brain. / De Seranno, Sandrine; Estrella, Cecilia; Loyens, Anne; Cornea, Anda; Ojeda, Sergio; Beauvillain, Jean Claude; Prevot, Vincent.

    In: Journal of Neuroscience, Vol. 24, No. 46, 17.11.2004, p. 10353-10363.

    Research output: Contribution to journalArticle

    De Seranno, S, Estrella, C, Loyens, A, Cornea, A, Ojeda, S, Beauvillain, JC & Prevot, V 2004, 'Vascular endothelial cells promote acute plasticity in ependymoglial cells of the neuroendocrine brain', Journal of Neuroscience, vol. 24, no. 46, pp. 10353-10363. https://doi.org/10.1523/JNEUROSCI.3228-04.2004
    De Seranno, Sandrine ; Estrella, Cecilia ; Loyens, Anne ; Cornea, Anda ; Ojeda, Sergio ; Beauvillain, Jean Claude ; Prevot, Vincent. / Vascular endothelial cells promote acute plasticity in ependymoglial cells of the neuroendocrine brain. In: Journal of Neuroscience. 2004 ; Vol. 24, No. 46. pp. 10353-10363.
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