Vascular endothelial cells promote acute plasticity in ependymoglial cells of the neuroendocrine brain

Sandrine De Seranno, Cecilia Estrella, Anne Loyens, Anda Cornea, Sergio R. Ojeda, Jean Claude Beauvillain, Vincent Prevot

    Research output: Contribution to journalArticle

    57 Scopus citations

    Abstract

    Glial and endothelial cells interact throughout the brain to define specific functional domains. Whether endothelial cells convey signals to glia in the mature brain is unknown but is amenable to examination in circumventricular organs. Here we report that purified endothelial cells of one of these organs, the median eminence of the hypothalamus, induce acute actin cytoskeleton remodeling in isolated ependymoglial cells and show that this plasticity is mediated by nitric oxide (NO), a diffusible factor. We found that both soluble guanylyl cyclase and cyclooxygenase products are involved in this endothelial-mediated control of ependymoglia cytoarchitecture. We also demonstrate by electron microscopy that activation of endogenous NO release in the median eminence induces rapid structural changes, allowing a direct access of neurosecretory axons containing gonadotropin-releasing hormone (GnRH) (the neuropeptide controlling reproductive function) to the portal vasculature. Local in vivo inhibition of NO synthesis disrupts reproductive cycliciry, a process that requires a pulsatile, coordinated delivery of GnRH into the hypothalamic-adenohypophyseal portal system. Our results identify a previously unknown function for endothelial cells in inducing neuroglial plasticity and raise the intriguing possibility that endothelial cells throughout the brain may use a similar signaling mechanism to regulate glial-neuronal interactions.

    Original languageEnglish (US)
    Pages (from-to)10353-10363
    Number of pages11
    JournalJournal of Neuroscience
    Volume24
    Issue number46
    DOIs
    StatePublished - Nov 17 2004

    Keywords

    • Endothelial nitric oxide synthase
    • Glial cells
    • Hypothalamus
    • Neuroglial plasticity
    • Neurosecretion
    • Tanycytes

    ASJC Scopus subject areas

    • Neuroscience(all)

    Fingerprint Dive into the research topics of 'Vascular endothelial cells promote acute plasticity in ependymoglial cells of the neuroendocrine brain'. Together they form a unique fingerprint.

  • Cite this