Vascular endothelial cells and granulopoiesis: Interleukin-1 stimulates release of G-CSF and GM-CSF

K. M. Zsebo, V. N. Yuschenkoff, S. Schiffer, D. Chang, E. McCall, C. A. Dinarello, M. A. Brown, B. Altrock, G. C. Bagby

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

Cultured mononuclear phagocytes produce soluble factors that stimulate endothelial cells to release GM-colony-stimulating activity (GM-CSA). One such factor was recently identified as interleukin 1 (IL 1). Studies were designed to determine which types of granulopoietic factors are released by IL 1-stimulated endothelial cells. Supernatants from endothelial cells cultured for 3 days in medium containing IL 1 α and β were tested in both murine and human CFU-GM colony growth assays. The effect of conditioned media on differentiation of WEHI-3B myelomonocytic leukemic cells was also examined. Control media containing IL 1 alone or unstimulated endothelial cell-conditioned media contained no detectable CSA in any bioassay. Medium conditioned by IL 1-stimulated endothelial cells stimulated the clonal growth of both human and murine CFU-GM and induced macrophage differentiation of WEHI-3B cells. Treatment of these conditioned media with a highly specific neutralizing monoclonal G-CSF antibody completely inhibited their activity in the murine CFU-GM assay, but only partially inhibited GM colony growth by human marrow. Treatment of the active conditioned media with a neutralizing rabbit anti-human GM-CSF antibody partially reduced the activity of the media in the human GM-colony growth assay. G-CSF radioimmunoassay of endothelial cell culture supernatants and Northern blot analysis of endothelial cell cytoplasmic RNA for GM-CSF gene transcripts confirmed that IL 1 iknduced expression of both G-CSF and GM-CSF genes. Because treatment of media with both antibodies abrogated all activity in the human GM colony growth assay, we conclude that IL 1-stimulated endothelial cells release both G and GM-CSF and that these are the only granulopoietic factors detectable in clonogenic assays released by these cells in vitro.

Original languageEnglish (US)
Pages (from-to)99-103
Number of pages5
JournalBlood
Volume71
Issue number1
StatePublished - 1988
Externally publishedYes

Fingerprint

Endothelial cells
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-1
Endothelial Cells
Conditioned Culture Medium
Assays
Granulocyte-Macrophage Progenitor Cells
Growth
Antibodies
Genes
Colony-Forming Units Assay
Bioassay
Macrophages
Phagocytes
Cell culture
Human Activities
Biological Assay
Northern Blotting
Radioimmunoassay

ASJC Scopus subject areas

  • Hematology

Cite this

Zsebo, K. M., Yuschenkoff, V. N., Schiffer, S., Chang, D., McCall, E., Dinarello, C. A., ... Bagby, G. C. (1988). Vascular endothelial cells and granulopoiesis: Interleukin-1 stimulates release of G-CSF and GM-CSF. Blood, 71(1), 99-103.

Vascular endothelial cells and granulopoiesis : Interleukin-1 stimulates release of G-CSF and GM-CSF. / Zsebo, K. M.; Yuschenkoff, V. N.; Schiffer, S.; Chang, D.; McCall, E.; Dinarello, C. A.; Brown, M. A.; Altrock, B.; Bagby, G. C.

In: Blood, Vol. 71, No. 1, 1988, p. 99-103.

Research output: Contribution to journalArticle

Zsebo, KM, Yuschenkoff, VN, Schiffer, S, Chang, D, McCall, E, Dinarello, CA, Brown, MA, Altrock, B & Bagby, GC 1988, 'Vascular endothelial cells and granulopoiesis: Interleukin-1 stimulates release of G-CSF and GM-CSF', Blood, vol. 71, no. 1, pp. 99-103.
Zsebo KM, Yuschenkoff VN, Schiffer S, Chang D, McCall E, Dinarello CA et al. Vascular endothelial cells and granulopoiesis: Interleukin-1 stimulates release of G-CSF and GM-CSF. Blood. 1988;71(1):99-103.
Zsebo, K. M. ; Yuschenkoff, V. N. ; Schiffer, S. ; Chang, D. ; McCall, E. ; Dinarello, C. A. ; Brown, M. A. ; Altrock, B. ; Bagby, G. C. / Vascular endothelial cells and granulopoiesis : Interleukin-1 stimulates release of G-CSF and GM-CSF. In: Blood. 1988 ; Vol. 71, No. 1. pp. 99-103.
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