Vascular addressins are induced on islet vessels during insulitis in nonobese diabetic mice and are involved in lymphoid cell binding to islet endothelium

Amo Hänninen, Cariel Taylor, Philip Streeter, Leslie S. Stark, Jaime M. Sarte, Judith A. Shizuru, Olli Simell, Sara A. Michie

Research output: Contribution to journalArticle

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Abstract

In the nonobese diabetic (NOD) mouse, lymphocytic and monocytic infiltration of the pancreatic islets leads to beta cell destruction. To investigate the mechanisms by which lymphocytes enter the NOD pancreas, pancreata were immunostained using monoclonal antibodies to a variety of adhesion molecules known to be involved in lymphocyte binding to vascular endothelium, an initial step in the migration of lymphocytes from blood into organized lymphoid and inflamed tissues. These adhesion molecules include: lymphocyte homing receptors involved in tissue-selective binding of lymphocytes to peripheral lymph node (L-selectin) or mucosal lymphoid tissue (LPAM-1, α4β7-integrin) high-endothelial venules (HEV); and HEV ligands peripheral vascular addressin (PNAd) and mucosal vascular addressin (MAdCAM-1). In NOD pancreata, α4β7, is expressed on most infiltrating cells at all stages of insulitis, whereas L-selectin expression is more pronounced on cells in the islets at later stages. During the development of insulitis, MAdCAM-1 and to a lesser extent PNAd became detectable on vascular endothelium adjacent to and within the inflamed islets. The Stamper-Woodruff in vitro assay was used to examine lymphoid cell binding to such vessels. These functional assays show that both the mucosal (MAdCAM-1/α4β7) and the peripheral (PNAd/L-selectin) recognition systems are involved in this binding. Our findings demonstrate that expression of peripheral and mucosal vascular addressins is induced on endothelium in inflamed islets in NOD pancreas, and that these addressins participate in binding lymphoid cells via their homing receptors. This suggests that these adhesion molecules play a role in the pathogenesis of diabetes in these mice by being involved in the migration of lymphocytes from blood into the inflamed pancreas.

Original languageEnglish (US)
Pages (from-to)2509-2515
Number of pages7
JournalJournal of Clinical Investigation
Volume92
Issue number5
StatePublished - 1993
Externally publishedYes

Fingerprint

Inbred NOD Mouse
Endothelium
Lymphocytes
Pancreas
L-Selectin
Venules
Vascular Endothelium
Lymphoid Tissue
Islets of Langerhans
Lymphocyte Homing Receptors
L-selectin counter-receptors
Integrins
Mucous Membrane
Lymph Nodes
Monoclonal Antibodies
Ligands

Keywords

  • Adhesion molecules
  • Inflammation
  • Insulin-dependent diabetes
  • Insulitis
  • NOD mouse

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hänninen, A., Taylor, C., Streeter, P., Stark, L. S., Sarte, J. M., Shizuru, J. A., ... Michie, S. A. (1993). Vascular addressins are induced on islet vessels during insulitis in nonobese diabetic mice and are involved in lymphoid cell binding to islet endothelium. Journal of Clinical Investigation, 92(5), 2509-2515.

Vascular addressins are induced on islet vessels during insulitis in nonobese diabetic mice and are involved in lymphoid cell binding to islet endothelium. / Hänninen, Amo; Taylor, Cariel; Streeter, Philip; Stark, Leslie S.; Sarte, Jaime M.; Shizuru, Judith A.; Simell, Olli; Michie, Sara A.

In: Journal of Clinical Investigation, Vol. 92, No. 5, 1993, p. 2509-2515.

Research output: Contribution to journalArticle

Hänninen, A, Taylor, C, Streeter, P, Stark, LS, Sarte, JM, Shizuru, JA, Simell, O & Michie, SA 1993, 'Vascular addressins are induced on islet vessels during insulitis in nonobese diabetic mice and are involved in lymphoid cell binding to islet endothelium', Journal of Clinical Investigation, vol. 92, no. 5, pp. 2509-2515.
Hänninen, Amo ; Taylor, Cariel ; Streeter, Philip ; Stark, Leslie S. ; Sarte, Jaime M. ; Shizuru, Judith A. ; Simell, Olli ; Michie, Sara A. / Vascular addressins are induced on islet vessels during insulitis in nonobese diabetic mice and are involved in lymphoid cell binding to islet endothelium. In: Journal of Clinical Investigation. 1993 ; Vol. 92, No. 5. pp. 2509-2515.
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AU - Stark, Leslie S.

AU - Sarte, Jaime M.

AU - Shizuru, Judith A.

AU - Simell, Olli

AU - Michie, Sara A.

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N2 - In the nonobese diabetic (NOD) mouse, lymphocytic and monocytic infiltration of the pancreatic islets leads to beta cell destruction. To investigate the mechanisms by which lymphocytes enter the NOD pancreas, pancreata were immunostained using monoclonal antibodies to a variety of adhesion molecules known to be involved in lymphocyte binding to vascular endothelium, an initial step in the migration of lymphocytes from blood into organized lymphoid and inflamed tissues. These adhesion molecules include: lymphocyte homing receptors involved in tissue-selective binding of lymphocytes to peripheral lymph node (L-selectin) or mucosal lymphoid tissue (LPAM-1, α4β7-integrin) high-endothelial venules (HEV); and HEV ligands peripheral vascular addressin (PNAd) and mucosal vascular addressin (MAdCAM-1). In NOD pancreata, α4β7, is expressed on most infiltrating cells at all stages of insulitis, whereas L-selectin expression is more pronounced on cells in the islets at later stages. During the development of insulitis, MAdCAM-1 and to a lesser extent PNAd became detectable on vascular endothelium adjacent to and within the inflamed islets. The Stamper-Woodruff in vitro assay was used to examine lymphoid cell binding to such vessels. These functional assays show that both the mucosal (MAdCAM-1/α4β7) and the peripheral (PNAd/L-selectin) recognition systems are involved in this binding. Our findings demonstrate that expression of peripheral and mucosal vascular addressins is induced on endothelium in inflamed islets in NOD pancreas, and that these addressins participate in binding lymphoid cells via their homing receptors. This suggests that these adhesion molecules play a role in the pathogenesis of diabetes in these mice by being involved in the migration of lymphocytes from blood into the inflamed pancreas.

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