Varied Length Stokes Shift BODIPY-Based Fluorophores for Multicolor Microscopy

Amy M. Bittel, Ashley M. Davis, Lei Wang, Michel A. Nederlof, Jorge O. Escobedo, Robert M. Strongin, Summer Gibbs

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Multicolor microscopy tools necessary to localize and visualize the complexity of subcellular systems are limited by current fluorophore technology. While commercial fluorophores cover spectral space from the ultraviolet to the near infrared region and are optimized for conventional bandpass based fluorescence microscopy, they are not ideal for highly multiplexed fluorescence microscopy as they tend to have short Stokes shifts, restricting the number of fluorophores that can be detected in a single sample to four to five. Herein, we synthesized a library of 95 novel boron-dipyrromethene (BODIPY)-based fluorophores and screened their photophysical, optical and spectral properties for their utility in multicolor microscopy. A subset of our BODIPY-based fluorophores yielded varied length Stokes shifts probes, which were used to create a five-color image using a single excitation with confocal laser scanning microscopy for the first time. Combining these novel fluorophores with conventional fluorophores could facilitate imaging in up to nine to ten colors using linear unmixing based microscopy approaches.

Original languageEnglish (US)
Article number4590
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Boron
Fluorophores
Microscopic examination
Fluorescence microscopy
Color
dipyrromethene
Infrared radiation
Scanning
Imaging techniques
Lasers

ASJC Scopus subject areas

  • General

Cite this

Bittel, A. M., Davis, A. M., Wang, L., Nederlof, M. A., Escobedo, J. O., Strongin, R. M., & Gibbs, S. (2018). Varied Length Stokes Shift BODIPY-Based Fluorophores for Multicolor Microscopy. Scientific Reports, 8(1), [4590]. https://doi.org/10.1038/s41598-018-22892-8

Varied Length Stokes Shift BODIPY-Based Fluorophores for Multicolor Microscopy. / Bittel, Amy M.; Davis, Ashley M.; Wang, Lei; Nederlof, Michel A.; Escobedo, Jorge O.; Strongin, Robert M.; Gibbs, Summer.

In: Scientific Reports, Vol. 8, No. 1, 4590, 01.12.2018.

Research output: Contribution to journalArticle

Bittel, AM, Davis, AM, Wang, L, Nederlof, MA, Escobedo, JO, Strongin, RM & Gibbs, S 2018, 'Varied Length Stokes Shift BODIPY-Based Fluorophores for Multicolor Microscopy', Scientific Reports, vol. 8, no. 1, 4590. https://doi.org/10.1038/s41598-018-22892-8
Bittel AM, Davis AM, Wang L, Nederlof MA, Escobedo JO, Strongin RM et al. Varied Length Stokes Shift BODIPY-Based Fluorophores for Multicolor Microscopy. Scientific Reports. 2018 Dec 1;8(1). 4590. https://doi.org/10.1038/s41598-018-22892-8
Bittel, Amy M. ; Davis, Ashley M. ; Wang, Lei ; Nederlof, Michel A. ; Escobedo, Jorge O. ; Strongin, Robert M. ; Gibbs, Summer. / Varied Length Stokes Shift BODIPY-Based Fluorophores for Multicolor Microscopy. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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