Variations in polyoma virus genotype in relation to tumor induction in mice. Characterization of wild type strains with widely differing tumor profiles

C. J. Dawe, R. Freund, Gail Mandel, K. Ballmer-Hofer, D. A. Talmage, T. L. Benjamin

Research output: Contribution to journalArticle

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Abstract

The authors have explored the effects of variations in mouse polyoma virus genotype on patterns of tumor formation in the mouse. Four 'wild type' virus strains were surveyed. Two were highly oncogenic, inducing multiple tumors of epithelial and mesenchymal origin, at high frequency and with short latency. The other two strains were weakly oncogenic, inducing fewer tumors, solely of mesenchymal origin, and after a long latency. These sharply contrasting tumor profiles were reproduced with virus stocks derived from molecularly cloned viral genomes. Though vastly different in their oncogenic properties, these cloned viruses proved equally effective in transforming established rat fibroblasts in culture and showed the same patterns of tumor antigen expression in cultured mouse cells. Complexes of polyoma middle T antigen and pp60(c-src) were demonstrated in extracts of epithelial tumors induced by a highly oncogenic virus strain. It is concluded that polyoma viral genetic determinants for tumor induction in the mouse are more complex than those previously defined by the use of cell transformation systems.

Original languageEnglish (US)
Pages (from-to)243-261
Number of pages19
JournalAmerican Journal of Pathology
Volume127
Issue number2
StatePublished - 1987
Externally publishedYes

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Polyomavirus
Genotype
Neoplasms
Viruses
Proto-Oncogene Proteins pp60(c-src)
Oncogenic Viruses
Viral Genome
Viral Tumor Antigens
Neoplasm Antigens
Cultured Cells
Fibroblasts

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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Variations in polyoma virus genotype in relation to tumor induction in mice. Characterization of wild type strains with widely differing tumor profiles. / Dawe, C. J.; Freund, R.; Mandel, Gail; Ballmer-Hofer, K.; Talmage, D. A.; Benjamin, T. L.

In: American Journal of Pathology, Vol. 127, No. 2, 1987, p. 243-261.

Research output: Contribution to journalArticle

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