Variation in GABA mini amplitude is the consequence of variation in transmitter concentration

Matthew Frerking, Salvador Borges, Martin Wilson

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Miniature postsynaptic currents (minis) in cultured retinal amacrine cells, as in other central neurons, show large variations in amplitude. To understand the origin of this variability, we have exploited a novel form of synapse in which pre- and postsynaptic receptors sample the same quantum of transmitter. At these synapses, mini amplitudes measured simultaneously in the 2 cells show a strong correlation, accounting for, on average, more than half of the variance in amplitude. Two pieces of evidence support the conclusion that variations in the amount of transmitter in different quanta underlie this correlation. First, diazepam, which enhances GABA binding, increases mini amplitude, implying therefore that transmitter concentration is not saturating. Second, we show that amplitude distributions from all cells, even those with a small number of release sites, have the same shape, implying that most or all variance is intrinsic to each release site.

Original languageEnglish (US)
Pages (from-to)885-895
Number of pages11
JournalNeuron
Volume15
Issue number4
DOIs
StatePublished - 1995
Externally publishedYes

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Synapses
gamma-Aminobutyric Acid
Presynaptic Receptors
Amacrine Cells
Synaptic Potentials
Diazepam
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Variation in GABA mini amplitude is the consequence of variation in transmitter concentration. / Frerking, Matthew; Borges, Salvador; Wilson, Martin.

In: Neuron, Vol. 15, No. 4, 1995, p. 885-895.

Research output: Contribution to journalArticle

Frerking, Matthew ; Borges, Salvador ; Wilson, Martin. / Variation in GABA mini amplitude is the consequence of variation in transmitter concentration. In: Neuron. 1995 ; Vol. 15, No. 4. pp. 885-895.
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