Variability of the 15N chemical shift anisotropy in Escherichia coli ribonuclease H in solution

Christopher D. Kroenke, Mark Rance, Arthur G. Palmer

Research output: Contribution to journalArticlepeer-review

127 Scopus citations

Abstract

The 15N chemical shift anisotropy, Δσ, is reported for 81 well- ordered backbone amide sites in the Escherichia coli enzyme ribonuclease H. The values of Δσ were determined from 15N relaxation rate constants measured at static magnetic field strengths of 11.7, 14.1, and 18.7 T; the data analyzed included both autorelaxation rate constants and 1H-15N dipolar/15N chemical shift anisotropy relaxation interference rate constants. For this data set, the values of Δσ are approximately Gaussian distributed with a mean of -172 ± 13 ppm. The standard deviation of the site-to-site variation of the chemical shift anisotropy is 5.5 ppm and a 95% confidence limit for this variation is 9.6 ppm. The site-to-site variation in the chemical shift anisotropy is similar to the variation observed in solid state NMR studies of specifically labeled polypeptides. Variability in the value of Δσ becomes a significant factor in the interpretation of spin relaxation rate constants at 18.7 T, but is less significant at lower field strengths, for the precision of relaxation data presently achievable by solution NMR. Ribonuclease H is the second protein for which an extensive set of Δσ values is available. Comparison of results for ribonuclease H with results reported for well-ordered sites in ubiquitin (Fushman, D.; Tjandra, N.; Cowburn, D. J. Am. Chem. Soc. 1998, 120, 10947-10952) reveals differences primarily in the larger number of statistically significant values far from the mean in ubiquitin.

Original languageEnglish (US)
Pages (from-to)10119-10125
Number of pages7
JournalJournal of the American Chemical Society
Volume121
Issue number43
DOIs
StatePublished - Nov 3 1999
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint

Dive into the research topics of 'Variability of the 15N chemical shift anisotropy in Escherichia coli ribonuclease H in solution'. Together they form a unique fingerprint.

Cite this