Vanilloid receptor expression and capsaicin excitation of rat dental primary afferent neurons

Priya Chaudhary, M. E. Martenson, T. K. Baumann

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Little is known about the molecular mechanisms that cause excitation of neurons which innervate the teeth. We investigated whether rat dental sensory neurons express the vanilloid (capsaicin) receptor (VR1). Dental sensory neurons were identified by retrograde transport of the fluorescent dye DiIC18 placed in maxillary molars. Patchclamp recordings in culture showed that 65% of DiIC18-labeled rat trigeminal ganglion neurons are excited by capsaicin. Responders covered the entire range of cell sizes examined (soma diameter, 24 to 48 μm). All non-responders had a soma diameter > 33 μm. Capsazepine (1 μM) reduced the capsaicin-evoked membrane current (6/6) and depolarization (7/7 responders). RT-PCR amplified a 375-bp product from DiIC18-labeled neurons which was identical to that expected for VR1. Thus, many rat dental primary afferent neurons are excited by capsaicin, and the response appears to be mediated by VR1. These results suggest that pharmacological blockers of VR1 may provide significant relief of dental pain.

Original languageEnglish (US)
Pages (from-to)1518-1523
Number of pages6
JournalJournal of Dental Research
Volume80
Issue number6
StatePublished - 2001

Fingerprint

TRPV Cation Channels
Afferent Neurons
Capsaicin
Tooth
Carisoprodol
Sensory Receptor Cells
Neurons
Trigeminal Ganglion
Fluorescent Dyes
Cell Size
Pharmacology
Pain
Polymerase Chain Reaction
Membranes

Keywords

  • Nociceptor
  • Patch clamp
  • RT-PCR, VR1
  • Sensory
  • Tooth
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Vanilloid receptor expression and capsaicin excitation of rat dental primary afferent neurons. / Chaudhary, Priya; Martenson, M. E.; Baumann, T. K.

In: Journal of Dental Research, Vol. 80, No. 6, 2001, p. 1518-1523.

Research output: Contribution to journalArticle

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