Vancomycin enhancement of experimental tobramycin nephrotoxicity

C. A. Wood, S. J. Kohlhepp, P. W. Kohnen, Donald Houghton, D. N. Gilbert

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The influence of vancomycin on tobramycin nephrotoxicity was assessed in male Fischer rats. Treatment groups included controls receiving diluent and grops receiving vancomycin alone at a dosage of 200 mg/kg (body weight) per day, tobramycin alone at dosage of 80 mg/kg per day, and a combination of vancomycin and tobramycin at the above dosages. All regimens were injected on a twice-a-day schedule. The animals were sacrificed on days 1, 3, 10, 14, 17 and 21. When compared with controls, animals receiving vancomycin alone exhibited no detectable renal toxicity. Compared with the case with controls, tobramycin alone was toxic, as manifested by lower mean animal weights, increased blood urea nitrogen concentrations on days 14 and 17 (P <0.005) increased serum creatinine concentrations on days 17 and 21 (P <0.005), and the presence of renal cortical tubular necrosis and regeneration. When compared with tobramycin alone, the combination of vancomycin and tobramycin caused earlier and more severe toxicity. By day 10, the magnitude of weight loss, the rise in blood urea nitrogen, and the increase in serum creatinine concentration were all greater in the rats given the combination of vancomycin plus tobramycin that in the animals given tobramycin alone (P <0.005). In addition, there was more proximal tubular necrosis and regeneration in rats given vancomycin plus tobramycin compared with those given tobramycin alone. In this animal model, vancomycin alone caused no detectable renal injury, tobramycin alone produced minimal proximal tubular damage, and the combination of vancomycin and tobramycin resulted in a greater degree of kidney injury than observed with tobramycin alone.

Original languageEnglish (US)
Pages (from-to)20-24
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume30
Issue number1
StatePublished - 1986
Externally publishedYes

Fingerprint

Tobramycin
Vancomycin
Blood Urea Nitrogen
Kidney
Regeneration
Creatinine
Kidney Cortex Necrosis
Poisons
Inbred F344 Rats
Wounds and Injuries
Serum
Weight Loss
Appointments and Schedules
Necrosis

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Wood, C. A., Kohlhepp, S. J., Kohnen, P. W., Houghton, D., & Gilbert, D. N. (1986). Vancomycin enhancement of experimental tobramycin nephrotoxicity. Antimicrobial Agents and Chemotherapy, 30(1), 20-24.

Vancomycin enhancement of experimental tobramycin nephrotoxicity. / Wood, C. A.; Kohlhepp, S. J.; Kohnen, P. W.; Houghton, Donald; Gilbert, D. N.

In: Antimicrobial Agents and Chemotherapy, Vol. 30, No. 1, 1986, p. 20-24.

Research output: Contribution to journalArticle

Wood, CA, Kohlhepp, SJ, Kohnen, PW, Houghton, D & Gilbert, DN 1986, 'Vancomycin enhancement of experimental tobramycin nephrotoxicity', Antimicrobial Agents and Chemotherapy, vol. 30, no. 1, pp. 20-24.
Wood, C. A. ; Kohlhepp, S. J. ; Kohnen, P. W. ; Houghton, Donald ; Gilbert, D. N. / Vancomycin enhancement of experimental tobramycin nephrotoxicity. In: Antimicrobial Agents and Chemotherapy. 1986 ; Vol. 30, No. 1. pp. 20-24.
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