Validation of fanconi anemia complementation group A assignment using molecular analysis

Nabil N. Moghrabi, Monique A. Johnson, Marvin J. Yoshitomi, Xiaoman Zhu, Muhsen J. Al-Dhalimy, Susan Olson, Markus Grompe, Carolyn (Sue) Richards

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: Fanconi anemia is a genetically heterogeneous chromosomal breakage disorder exhibiting a high degree of clinical variability Clinical diagn oses are confirmed by testing patient cells for increased sensitivity to crosslinking agents Fanconi anemia complementation group assignment, essential for efficient molecular diagnosis of the disease, had not been validated for clinical application before this study The purpose of this study was (1) confirmation of the accuracy of Fanconi anemia complementation group assignment to Group A (FANCA) and (2) development of a rapid mutation detection strategy that ensures the efficient capture of all FANCA mutations Methods: Using fibroblasts from 29 patients, diagnosis of Fanconi anemia and assignment to complementation Group A was made through breakage analysis studies FANCA coding and flanking sequences were analyzed using denaturing high pressure liquid chromatography, sequencing, and multiplex ligation-dependent probe amplification Patients in which two mutations were not identified were analyzed by cDNA sequencing Patients with no mutations were sequenced for mutations in FANCC, G, E, and F Results: Of the 56 putative mutant alleles studied, 89% had an identifiable FANCA pathogenic mutation Eight unique novel mutations were identified Conclusion: Complementation assignment to Group A was validated in a clinical laboratory setting using our FANCA rapid molecular testing strategy.

Original languageEnglish (US)
Pages (from-to)183-192
Number of pages10
JournalGenetics in Medicine
Volume11
Issue number3
DOIs
StatePublished - Mar 2009

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Fanconi Anemia
Mutation
Chromosome Disorders
Chromosome Breakage
Multiplex Polymerase Chain Reaction
Complementary DNA
Fibroblasts
Alleles
High Pressure Liquid Chromatography

Keywords

  • Complementation group assignment
  • FANCA
  • Fanconi anemia
  • Molecular FANCA testing

ASJC Scopus subject areas

  • Genetics(clinical)

Cite this

Validation of fanconi anemia complementation group A assignment using molecular analysis. / Moghrabi, Nabil N.; Johnson, Monique A.; Yoshitomi, Marvin J.; Zhu, Xiaoman; Al-Dhalimy, Muhsen J.; Olson, Susan; Grompe, Markus; Richards, Carolyn (Sue).

In: Genetics in Medicine, Vol. 11, No. 3, 03.2009, p. 183-192.

Research output: Contribution to journalArticle

Moghrabi, Nabil N. ; Johnson, Monique A. ; Yoshitomi, Marvin J. ; Zhu, Xiaoman ; Al-Dhalimy, Muhsen J. ; Olson, Susan ; Grompe, Markus ; Richards, Carolyn (Sue). / Validation of fanconi anemia complementation group A assignment using molecular analysis. In: Genetics in Medicine. 2009 ; Vol. 11, No. 3. pp. 183-192.
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