Abstract
There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 55-59 |
| Number of pages | 5 |
| Journal | Journal of Chromatography A |
| Volume | 1210 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 7 2008 |
Keywords
- 3-Iodothyronamine
- LC-MS/MS
- Serum
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
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Cite this
DeBarber, A. E., Geraci, T., Colasurdo, V. P., Hackenmueller, S. A., & Scanlan, T. S. (2008). Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum. Journal of Chromatography A, 1210(1), 55-59. https://doi.org/10.1016/j.chroma.2008.09.022