Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum

Andrea E. DeBarber, Travis Geraci, Vincent P. Colasurdo, Sarah A. Hackenmueller, Thomas S. Scanlan

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.

Original languageEnglish (US)
Pages (from-to)55-59
Number of pages5
JournalJournal of Chromatography A
Volume1210
Issue number1
DOIs
StatePublished - Nov 7 2008

Keywords

  • 3-Iodothyronamine
  • LC-MS/MS
  • Serum

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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