Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum

Andrea De Barber; Travis Geraci; Vincent P. Colasurdo; Sarah A. Hackenmueller; Thomas (Tom) Scanlan

doi:10.1016/j.chroma.2008.09.022

Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum

Andrea De Barber, Travis Geraci, Vincent P. Colasurdo, Sarah A. Hackenmueller, Thomas (Tom) Scanlan

Physiology & Pharmacology

Research output: Contribution to journal › Article

15 Scopus citations

Abstract

There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.

Original language	English (US)
Pages (from-to)	55-59
Number of pages	5
Journal	Journal of Chromatography A
Volume	1210
Issue number	1
DOIs	https://doi.org/10.1016/j.chroma.2008.09.022
Publication status	Published - Nov 7 2008

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Keywords

3-Iodothyronamine
LC-MS/MS
Serum

ASJC Scopus subject areas

Analytical Chemistry
Organic Chemistry
Biochemistry

Cite this

De Barber, A., Geraci, T., Colasurdo, V. P., Hackenmueller, S. A., & Scanlan, T. T. (2008). Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum. Journal of Chromatography A, 1210(1), 55-59. https://doi.org/10.1016/j.chroma.2008.09.022

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abstract = "There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.",

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AU - Hackenmueller, Sarah A.

AU - Scanlan, Thomas (Tom)

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10.1016/j.chroma.2008.09.022