Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum

Andrea E. DeBarber; Travis Geraci; Vincent P. Colasurdo; Sarah A. Hackenmueller; Thomas S. Scanlan

doi:10.1016/j.chroma.2008.09.022

Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum

Andrea E. DeBarber, Travis Geraci, Vincent P. Colasurdo, Sarah A. Hackenmueller, Thomas S. Scanlan

Physiology & Pharmacology

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.

Original language	English (US)
Pages (from-to)	55-59
Number of pages	5
Journal	Journal of Chromatography A
Volume	1210
Issue number	1
DOIs	https://doi.org/10.1016/j.chroma.2008.09.022
State	Published - Nov 7 2008

Keywords

3-Iodothyronamine
LC-MS/MS
Serum

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum. / DeBarber, Andrea E.; Geraci, Travis; Colasurdo, Vincent P.; Hackenmueller, Sarah A.; Scanlan, Thomas S.

In: Journal of Chromatography A, Vol. 1210, No. 1, 07.11.2008, p. 55-59.

Research output: Contribution to journal › Article › peer-review

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title = "Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum",

abstract = "There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.",

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author = "DeBarber, {Andrea E.} and Travis Geraci and Colasurdo, {Vincent P.} and Hackenmueller, {Sarah A.} and Scanlan, {Thomas S.}",

note = "Funding Information: This work was supported by a NIH grant (DK-52798 to T.S.S.) and was accomplished using instrumentation housed in the Department of Physiology and Pharmacology Bioanalytical Shared Resource and Department of Chemistry Mass Spectrometry Facility at Portland State University, Portland, OR. We thank Chris Toombs at Ikaria, Inc. for providing serum from T 1 AM treated rats. ",

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AU - Hackenmueller, Sarah A.

AU - Scanlan, Thomas S.

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AB - There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.

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