Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum

Andrea De Barber, Travis Geraci, Vincent P. Colasurdo, Sarah A. Hackenmueller, Thomas (Tom) Scanlan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.

Original languageEnglish (US)
Pages (from-to)55-59
Number of pages5
JournalJournal of Chromatography A
Volume1210
Issue number1
DOIs
StatePublished - Nov 7 2008

Fingerprint

Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Serum
Bioactivity
Limit of Detection
Rats
Rodentia
Blood
3-iodothyronamine
Availability
Pharmacology
Tissue

Keywords

  • 3-Iodothyronamine
  • LC-MS/MS
  • Serum

ASJC Scopus subject areas

  • Analytical Chemistry
  • Organic Chemistry
  • Biochemistry

Cite this

Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum. / De Barber, Andrea; Geraci, Travis; Colasurdo, Vincent P.; Hackenmueller, Sarah A.; Scanlan, Thomas (Tom).

In: Journal of Chromatography A, Vol. 1210, No. 1, 07.11.2008, p. 55-59.

Research output: Contribution to journalArticle

De Barber, Andrea ; Geraci, Travis ; Colasurdo, Vincent P. ; Hackenmueller, Sarah A. ; Scanlan, Thomas (Tom). / Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum. In: Journal of Chromatography A. 2008 ; Vol. 1210, No. 1. pp. 55-59.
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