Abstract
There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 55-59 |
| Number of pages | 5 |
| Journal | Journal of Chromatography A |
| Volume | 1210 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 7 2008 |
Fingerprint
Keywords
- 3-Iodothyronamine
- LC-MS/MS
- Serum
ASJC Scopus subject areas
- Analytical Chemistry
- Organic Chemistry
- Biochemistry
Cite this
Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum. / De Barber, Andrea; Geraci, Travis; Colasurdo, Vincent P.; Hackenmueller, Sarah A.; Scanlan, Thomas (Tom).
In: Journal of Chromatography A, Vol. 1210, No. 1, 07.11.2008, p. 55-59.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine from serum
AU - De Barber, Andrea
AU - Geraci, Travis
AU - Colasurdo, Vincent P.
AU - Hackenmueller, Sarah A.
AU - Scanlan, Thomas (Tom)
PY - 2008/11/7
Y1 - 2008/11/7
N2 - There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.
AB - There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.
KW - 3-Iodothyronamine
KW - LC-MS/MS
KW - Serum
UR - http://www.scopus.com/inward/record.url?scp=53649093772&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=53649093772&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2008.09.022
DO - 10.1016/j.chroma.2008.09.022
M3 - Article
C2 - 18823891
AN - SCOPUS:53649093772
VL - 1210
SP - 55
EP - 59
JO - Journal of Chromatography
JF - Journal of Chromatography
SN - 0021-9673
IS - 1
ER -