Vaccinia virus systems for expression of Gα genes in S49 cells

Franklin Quan, Michael Forte

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

In this chapter, we describe the use of recombinant VV to express Gα subunit efficiently in S49 cyc cells, a murine lymphoma cell line deficient in endogenous Gsα activity. Additional studies have shown that VV infection does not interfere with G-protein-coupled signal transduction events.6 In particular, the VV-expressed rat Gsα subunit is able to mediate efficient receptor-dependent and receptor-independent activation of adenylyl cyclase in cyc cells. This system should therefore be useful for rapidly assessing the activity of modified or chimeric Gsα subunits.

Original languageEnglish (US)
Pages (from-to)436-445
Number of pages10
JournalMethods in Enzymology
Volume237
Issue numberC
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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