Spinal cord (SC) T cells were isolated at the onset of actively induced experimental autoimmune encephalomyelitis (EAE) and sorted for the presence of the OX-40 activation marker. Previously, we reported an enhanced bias in Vβ8.2 expression as well as enhanced proliferative responses to basic protein antigens among the OX-40+ SC T cells. Here we demonstrate that CDR3 motifs associated with EAE are present at a significantly higher frequency in Vβ8.2 sequences of OX-40+ SC T cells (16/17) compared with those of OX- 40- SC T cells (5/17). Thus, the OX-40 antigen may be useful as a marker to isolate and characterize autoantigen-specific T cells from the site of inflammation in T-cell-mediated autoimmune diseases.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Neuroscience Research|
|State||Published - Jun 15 1996|
- TCR Vβ
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience