Vβ CDR3 motifs associated with BP recognition are enriched in OX-40+ spinal cord T cells of Lewis rats with EAE

A. C. Buenafe; A. D. Weinberg; N. E. Culbertson; A. A. Vandenbark; H. Offner

doi:10.1002/(SICI)1097-4547(19960615)44:6<562::AID-JNR6>3.0.CO;2-9

Vβ CDR3 motifs associated with BP recognition are enriched in OX-40⁺ spinal cord T cells of Lewis rats with EAE

A. C. Buenafe, A. D. Weinberg, N. E. Culbertson, A. A. Vandenbark, H. Offner

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Spinal cord (SC) T cells were isolated at the onset of actively induced experimental autoimmune encephalomyelitis (EAE) and sorted for the presence of the OX-40 activation marker. Previously, we reported an enhanced bias in Vβ8.2 expression as well as enhanced proliferative responses to basic protein antigens among the OX-40⁺ SC T cells. Here we demonstrate that CDR3 motifs associated with EAE are present at a significantly higher frequency in Vβ8.2 sequences of OX-40⁺ SC T cells (16/17) compared with those of OX- 40^- SC T cells (5/17). Thus, the OX-40 antigen may be useful as a marker to isolate and characterize autoantigen-specific T cells from the site of inflammation in T-cell-mediated autoimmune diseases.

Original language	English (US)
Pages (from-to)	562-567
Number of pages	6
Journal	Journal of Neuroscience Research
Volume	44
Issue number	6
DOIs	https://doi.org/10.1002/(SICI)1097-4547(19960615)44:6<562::AID-JNR6>3.0.CO;2-9
State	Published - Jun 15 1996

Keywords

MS
TCR Vβ
autoimmunity

ASJC Scopus subject areas

Cellular and Molecular Neuroscience

Access to Document

10.1002/(SICI)1097-4547(19960615)44:6<562::AID-JNR6>3.0.CO;2-9

Cite this

@article{e452d77978864740aaa95f7a6e54e0a7,

title = "Vβ CDR3 motifs associated with BP recognition are enriched in OX-40+ spinal cord T cells of Lewis rats with EAE",

abstract = "Spinal cord (SC) T cells were isolated at the onset of actively induced experimental autoimmune encephalomyelitis (EAE) and sorted for the presence of the OX-40 activation marker. Previously, we reported an enhanced bias in Vβ8.2 expression as well as enhanced proliferative responses to basic protein antigens among the OX-40+ SC T cells. Here we demonstrate that CDR3 motifs associated with EAE are present at a significantly higher frequency in Vβ8.2 sequences of OX-40+ SC T cells (16/17) compared with those of OX- 40- SC T cells (5/17). Thus, the OX-40 antigen may be useful as a marker to isolate and characterize autoantigen-specific T cells from the site of inflammation in T-cell-mediated autoimmune diseases.",

keywords = "MS, TCR Vβ, autoimmunity",

author = "Buenafe, {A. C.} and Weinberg, {A. D.} and Culbertson, {N. E.} and Vandenbark, {A. A.} and H. Offner",

year = "1996",

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T1 - Vβ CDR3 motifs associated with BP recognition are enriched in OX-40+ spinal cord T cells of Lewis rats with EAE

AU - Buenafe, A. C.

AU - Weinberg, A. D.

AU - Culbertson, N. E.

AU - Vandenbark, A. A.

AU - Offner, H.

PY - 1996/6/15

Y1 - 1996/6/15

N2 - Spinal cord (SC) T cells were isolated at the onset of actively induced experimental autoimmune encephalomyelitis (EAE) and sorted for the presence of the OX-40 activation marker. Previously, we reported an enhanced bias in Vβ8.2 expression as well as enhanced proliferative responses to basic protein antigens among the OX-40+ SC T cells. Here we demonstrate that CDR3 motifs associated with EAE are present at a significantly higher frequency in Vβ8.2 sequences of OX-40+ SC T cells (16/17) compared with those of OX- 40- SC T cells (5/17). Thus, the OX-40 antigen may be useful as a marker to isolate and characterize autoantigen-specific T cells from the site of inflammation in T-cell-mediated autoimmune diseases.

AB - Spinal cord (SC) T cells were isolated at the onset of actively induced experimental autoimmune encephalomyelitis (EAE) and sorted for the presence of the OX-40 activation marker. Previously, we reported an enhanced bias in Vβ8.2 expression as well as enhanced proliferative responses to basic protein antigens among the OX-40+ SC T cells. Here we demonstrate that CDR3 motifs associated with EAE are present at a significantly higher frequency in Vβ8.2 sequences of OX-40+ SC T cells (16/17) compared with those of OX- 40- SC T cells (5/17). Thus, the OX-40 antigen may be useful as a marker to isolate and characterize autoantigen-specific T cells from the site of inflammation in T-cell-mediated autoimmune diseases.

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