Utility of a combination of RD1 and RD2 antigens as a diagnostic marker for tuberculosis

Mamta Kalra, Gopal Krishan Khuller, Ajay Grover, Digambar Behera, Ajay Wanchu, Indu Verma

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

We evaluated the diagnostic potential of a cocktail of 4 antigens encoded by regions of difference (RD) 1 and 2 of Mycobacterium tuberculosis, that is, early secretory antigenic target-6, culture filtrate protein-10 (CFP-10), CFP-21, and mycobacterial protein from species tuberculosis-64 (MPT-64) on the basis of antigen and antibody detection by enzyme-linked immunosorbent assay. Parallel detection of antigens and antibodies in the serum samples of pulmonary tuberculosis (PTB) patients resulted in higher sensitivity as compared to either of the single tests in both smear-positive (90%) and smear-negative (60%) PTB patients. In addition, combined detection of antigens and antibodies in the fluids of extrapulmonary tuberculosis (EPTB) patients could detect >90% of the patients with high specificity. These results demonstrate the ability of the combination of antigen and antibody detection assays based on the cocktail of RD antigens to diagnose a substantial number of PTB and EPTB cases with high specificity.

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalDiagnostic Microbiology and Infectious Disease
Volume66
Issue number2
DOIs
StatePublished - Feb 1 2010

Keywords

  • Antibody detection
  • Antigen detection
  • ELISA
  • RD antigens
  • Tuberculosis

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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