Uterine metabolic activity and steroid receptor concentrations in response to suppressed secretion of PRL in anestrous mink

Ov Slayden, Fredrick Stormshak

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Two experiments were conducted to evaluate the effects of bromocriptine, melatonin (MLT), and 17β-estradiol (E) on uterine physiology in mink (Mustela vison). In Expt. 1, summer-anestrous mink were injected sc daily with 2 mg bromocriptine or vehicle (n = 20 each) for 14 days. On Day 14, both groups were divided into two subgroups and injected sc with either 100 μg E2 or vehicle. Mink were bled immediately prior to euthanasia (24 hr after E2) and the sera analyzed for prolactin (PRL), E2, and progesterone (P4). At necropsy, aliquots of uterine tissue (n = 5) were used to measure in vitro oxidation of [14C]glucose, incorporation of [3H]thymidine into DNA and [14C]leucine into protein, and nuclear concentrations of estrogen receptor (ER) and P4 receptor (PR). In Expt. 2, anestrous mink were assigned to one of two treatment groups or a control group (n = 5 each). In mid-summer, groups 1 and 2 were implanted with 10 mg Silastic MLT implants. Seventeen weeks later, mink in group 1 received 100 μg E2 (sc) while group 2 and nonimplanted controls (group 3) were injected with vehicle. Mink were sacrificed 24 hr after injection and levels of PRL, E2, P4, ER, and PR determined. Bromocriptine suppressed serum concentrations of PRL (P <0.001), increased-serum levels of E2 (P <0.05) and levels of PR (P <0.01), but had no effect on levels of P4, uterine weight, glucose oxidation, DNA and protein synthesis, or concentrations of ER. Treatment with E2 or bromocriptine plus E2 increased uterine weight (P <0.01), DNA and protein synthesis (P <0.01), and concentrations of ER (P <0.01) and PR (P <0.01). Exogenous MLT reduced serum levels of PRL (P <0.01) and increased concentrations of E2 (P <0.01). Both MLT and MLT plus E2 increased uterine weight (P <0.001) and levels of ER and PR (P <0.01). Results of this study indicate that inhibition of PRL secretion in anestrous mink increases endogenous levels of E2 which evoke uterine responses similar to those observed after treatment with exogenous E2.

Original languageEnglish (US)
Pages (from-to)307-315
Number of pages9
JournalGeneral and Comparative Endocrinology
Volume88
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Mink
Steroid Receptors
mink
prolactin
Prolactin
bromocriptine
melatonin
secretion
Melatonin
Estrogen Receptors
Bromocriptine
receptors
Serum
Weights and Measures
DNA
protein synthesis
oxidation
uterine tissue
Neovison vison
glucose

ASJC Scopus subject areas

  • Endocrinology

Cite this

Uterine metabolic activity and steroid receptor concentrations in response to suppressed secretion of PRL in anestrous mink. / Slayden, Ov; Stormshak, Fredrick.

In: General and Comparative Endocrinology, Vol. 88, No. 2, 1992, p. 307-315.

Research output: Contribution to journalArticle

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abstract = "Two experiments were conducted to evaluate the effects of bromocriptine, melatonin (MLT), and 17β-estradiol (E) on uterine physiology in mink (Mustela vison). In Expt. 1, summer-anestrous mink were injected sc daily with 2 mg bromocriptine or vehicle (n = 20 each) for 14 days. On Day 14, both groups were divided into two subgroups and injected sc with either 100 μg E2 or vehicle. Mink were bled immediately prior to euthanasia (24 hr after E2) and the sera analyzed for prolactin (PRL), E2, and progesterone (P4). At necropsy, aliquots of uterine tissue (n = 5) were used to measure in vitro oxidation of [14C]glucose, incorporation of [3H]thymidine into DNA and [14C]leucine into protein, and nuclear concentrations of estrogen receptor (ER) and P4 receptor (PR). In Expt. 2, anestrous mink were assigned to one of two treatment groups or a control group (n = 5 each). In mid-summer, groups 1 and 2 were implanted with 10 mg Silastic MLT implants. Seventeen weeks later, mink in group 1 received 100 μg E2 (sc) while group 2 and nonimplanted controls (group 3) were injected with vehicle. Mink were sacrificed 24 hr after injection and levels of PRL, E2, P4, ER, and PR determined. Bromocriptine suppressed serum concentrations of PRL (P <0.001), increased-serum levels of E2 (P <0.05) and levels of PR (P <0.01), but had no effect on levels of P4, uterine weight, glucose oxidation, DNA and protein synthesis, or concentrations of ER. Treatment with E2 or bromocriptine plus E2 increased uterine weight (P <0.01), DNA and protein synthesis (P <0.01), and concentrations of ER (P <0.01) and PR (P <0.01). Exogenous MLT reduced serum levels of PRL (P <0.01) and increased concentrations of E2 (P <0.01). Both MLT and MLT plus E2 increased uterine weight (P <0.001) and levels of ER and PR (P <0.01). Results of this study indicate that inhibition of PRL secretion in anestrous mink increases endogenous levels of E2 which evoke uterine responses similar to those observed after treatment with exogenous E2.",
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