TY - JOUR
T1 - Uterine metabolic activity and steroid receptor concentrations in response to suppressed secretion of PRL in anestrous mink
AU - Slayden, Ov
AU - Stormshak, Fredrick
N1 - Funding Information:
We gratefully acknowledge Dr. R. L. Butcher, West Virginia University Medical Center, for the donation of Ez specific antiserum (anti E,-3-HSA); Dr. G. D. Niswender, Colorado State University, for the P, antibody (#337 anti-progesterone-11-BSA), and Dr. D. Bolt, USDA, for donation of the highly purified pPRL (pPRL-I-2; AFP-5000). The authors also thank Ron Scott and Cliff Thomson of the Experimental Fur Farm at Oregon State University, for their care of the animals. This research was supported in part by the Mink Farmers’ Research Foundation, and the Olympic Fur Breeders Association.
PY - 1992/11
Y1 - 1992/11
N2 - Two experiments were conducted to evaluate the effects of bromocriptine, melatonin (MLT), and 17β-estradiol (E) on uterine physiology in mink (Mustela vison). In Expt. 1, summer-anestrous mink were injected sc daily with 2 mg bromocriptine or vehicle (n = 20 each) for 14 days. On Day 14, both groups were divided into two subgroups and injected sc with either 100 μg E2 or vehicle. Mink were bled immediately prior to euthanasia (24 hr after E2) and the sera analyzed for prolactin (PRL), E2, and progesterone (P4). At necropsy, aliquots of uterine tissue (n = 5) were used to measure in vitro oxidation of [14C]glucose, incorporation of [3H]thymidine into DNA and [14C]leucine into protein, and nuclear concentrations of estrogen receptor (ER) and P4 receptor (PR). In Expt. 2, anestrous mink were assigned to one of two treatment groups or a control group (n = 5 each). In mid-summer, groups 1 and 2 were implanted with 10 mg Silastic MLT implants. Seventeen weeks later, mink in group 1 received 100 μg E2 (sc) while group 2 and nonimplanted controls (group 3) were injected with vehicle. Mink were sacrificed 24 hr after injection and levels of PRL, E2, P4, ER, and PR determined. Bromocriptine suppressed serum concentrations of PRL (P < 0.001), increased-serum levels of E2 (P < 0.05) and levels of PR (P < 0.01), but had no effect on levels of P4, uterine weight, glucose oxidation, DNA and protein synthesis, or concentrations of ER. Treatment with E2 or bromocriptine plus E2 increased uterine weight (P < 0.01), DNA and protein synthesis (P < 0.01), and concentrations of ER (P < 0.01) and PR (P < 0.01). Exogenous MLT reduced serum levels of PRL (P < 0.01) and increased concentrations of E2 (P < 0.01). Both MLT and MLT plus E2 increased uterine weight (P < 0.001) and levels of ER and PR (P < 0.01). Results of this study indicate that inhibition of PRL secretion in anestrous mink increases endogenous levels of E2 which evoke uterine responses similar to those observed after treatment with exogenous E2.
AB - Two experiments were conducted to evaluate the effects of bromocriptine, melatonin (MLT), and 17β-estradiol (E) on uterine physiology in mink (Mustela vison). In Expt. 1, summer-anestrous mink were injected sc daily with 2 mg bromocriptine or vehicle (n = 20 each) for 14 days. On Day 14, both groups were divided into two subgroups and injected sc with either 100 μg E2 or vehicle. Mink were bled immediately prior to euthanasia (24 hr after E2) and the sera analyzed for prolactin (PRL), E2, and progesterone (P4). At necropsy, aliquots of uterine tissue (n = 5) were used to measure in vitro oxidation of [14C]glucose, incorporation of [3H]thymidine into DNA and [14C]leucine into protein, and nuclear concentrations of estrogen receptor (ER) and P4 receptor (PR). In Expt. 2, anestrous mink were assigned to one of two treatment groups or a control group (n = 5 each). In mid-summer, groups 1 and 2 were implanted with 10 mg Silastic MLT implants. Seventeen weeks later, mink in group 1 received 100 μg E2 (sc) while group 2 and nonimplanted controls (group 3) were injected with vehicle. Mink were sacrificed 24 hr after injection and levels of PRL, E2, P4, ER, and PR determined. Bromocriptine suppressed serum concentrations of PRL (P < 0.001), increased-serum levels of E2 (P < 0.05) and levels of PR (P < 0.01), but had no effect on levels of P4, uterine weight, glucose oxidation, DNA and protein synthesis, or concentrations of ER. Treatment with E2 or bromocriptine plus E2 increased uterine weight (P < 0.01), DNA and protein synthesis (P < 0.01), and concentrations of ER (P < 0.01) and PR (P < 0.01). Exogenous MLT reduced serum levels of PRL (P < 0.01) and increased concentrations of E2 (P < 0.01). Both MLT and MLT plus E2 increased uterine weight (P < 0.001) and levels of ER and PR (P < 0.01). Results of this study indicate that inhibition of PRL secretion in anestrous mink increases endogenous levels of E2 which evoke uterine responses similar to those observed after treatment with exogenous E2.
UR - http://www.scopus.com/inward/record.url?scp=0026706376&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026706376&partnerID=8YFLogxK
U2 - 10.1016/0016-6480(92)90264-K
DO - 10.1016/0016-6480(92)90264-K
M3 - Article
C2 - 1478446
AN - SCOPUS:0026706376
SN - 0016-6480
VL - 88
SP - 307
EP - 315
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 2
ER -