Using Reverse Phase Protein Array (RPPA) to Identify and Target Adaptive Resistance

Marilyne Labrie, Yong Fang, Nicholas D. Kendsersky, Jun Li, Han Liang, Shannon N. Westin, Zahi Mitri, Gordon B. Mills

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    Tumor cells and the tumor ecosystem rapidly evolve in response to therapy. This tumor evolution results in the rapid emergence of drug resistance that limits the magnitude and duration of response to therapy including chemotherapy, targeted therapy, and immunotherapy. Thus, there is an urgent need to understand and interdict tumor evolution to improve patient benefit to therapy. Reverse phase protein array (RPPA) provides a powerful tool to evaluate and develop approaches to target the processes underlying one form of tumor evolution: adaptive evolution. Tumor cells and the tumor microenvironment rapidly evolve through rewiring of protein networks to bypass the effects of therapy. In this review, we present the concepts underlying adaptive resistance and use of RPPA in understanding resistance mechanisms and identification of effective drug combinations. We further demonstrate that this novel information is resulting in biomarker-driven trials aimed at targeting adaptive resistance and improving patient outcomes.

    Original languageEnglish (US)
    Title of host publicationAdvances in Experimental Medicine and Biology
    PublisherSpringer
    Pages251-266
    Number of pages16
    DOIs
    StatePublished - Jan 1 2019

    Publication series

    NameAdvances in Experimental Medicine and Biology
    Volume1188
    ISSN (Print)0065-2598
    ISSN (Electronic)2214-8019

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    Keywords

    • Adaptive resistance
    • Combination therapy
    • Reverse phase protein array
    • Targeted therapy
    • Tumor evolution

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Labrie, M., Fang, Y., Kendsersky, N. D., Li, J., Liang, H., Westin, S. N., Mitri, Z., & Mills, G. B. (2019). Using Reverse Phase Protein Array (RPPA) to Identify and Target Adaptive Resistance. In Advances in Experimental Medicine and Biology (pp. 251-266). (Advances in Experimental Medicine and Biology; Vol. 1188). Springer. https://doi.org/10.1007/978-981-32-9755-5_14