Use of synthetic polymers improves the quality of vitrified caprine preantral follicles in the ovarian tissue

Diego Alberto Montano Vizcarra, Yago Pinto Silva, Jamily Bezerra Bruno, Danielle Cristina Calado Brito, Deysi Dipaz Berrocal, Luciana Mascena Silva, Maria Luana Gaudencio dos Santos Morais, Benner Geraldo Alves, Kele Amaral Alves, Francielli Weber Santos Cibin, José Ricardo Figueiredo, Mary B. Zelinski, Ana Paula Ribeiro Rodrigues

    Research output: Contribution to journalArticlepeer-review

    2 Scopus citations

    Abstract

    The aim of this study was to evaluate whether the addition of synthetic polymers to the vitrification solution affected follicular morphology and development and the expression of Ki-67, Aquaporin 3 (AQP3) and cleaved Caspase-3 proteins in ovarian tissue of the caprine species. Caprine ovaries were fragmented and two fragments were immediately fixed (Fresh Control) for morphological evaluation, while other two were in vitro cultured for 7 days (Cultured Control) and fixed as well. The remaining fragments were distributed in two different vitrification groups: Vitrified and Vitrified/Cultured. Each group was composed of 4 different treatments: 1) Sucrose (SUC); 2) SuperCool X-1000 0.2 % (X-1000); 3) SuperCool Z-1000 0.4 % (Z-1000) or 4) with polyvinylpyrrolidone K-12 0.2 % (PVP). Also, Fresh Control, Cultured Control, SUC and X-1000 were destined to immunohistochemical detection of Ki-67, AQP3 and cleaved Caspase-3 proteins. Morphologically, the treatment with X-1000 showed no significant difference with the Fresh Control group and was superior to the other treatments. After the cleaved caspase-3 analysis, X-1000 showed the lowest percentages of strong immunostaining while Cultured Control showed the highest. Also, a positive correlation was found between the percentages of degenerated follicles and the percentages of strong staining intensity follicles. Regarding the AQP3 analysis, the highest percentages of strong AQP3 staining intensity were found in X-1000. In conclusion, we have demonstrated that the addition of the synthetic polymer SuperCool X-1000 to the vitrification solution improved the current vitrification protocol of caprine ovarian tissue.

    Original languageEnglish (US)
    Article number151484
    JournalActa Histochemica
    Volume122
    Issue number2
    DOIs
    StatePublished - Feb 2020

    Keywords

    • In vitro culture
    • Polyvinylpyrrolidone
    • SuperCool X-1000
    • SuperCool Z-1000
    • Vitrification

    ASJC Scopus subject areas

    • Histology
    • Cell Biology

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