Use of polymerase chain reaction for successful identification of asymptomatic genital infection with herpes simplex virus in pregnant women at delivery

Deborah A. Hardy, Ann M. Arvin, Linda L. Yasukawa, Rachel N. Bronzan, David Lewinsohn, Paul A. Hensleigh, Charles G. Prober

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The polymerase chain reaction was adapted to the amplification of a herpes simplex virus (HSV) DNA sequence, common to HSV types 1 and 2 (HSV-1, HSV-2). The amplified product was detectable by ethidium-bromide staining or Southern hybridization of gels and by dot hybridization. The HSV polymerase chain reaction detected HSV DNA in samples obtained from eight patients with genital lesions from which HSV-2 was isolated in tissue culture and from four patients with labial lesions from which HSV-1 was isolated. The HSV polymerase chain reaction identified HSV in clinical specimens obtained from 11 women who had asymptomatic genital HSV infections at delivery. None of 11 samples obtained at delivery from women who had antibodies to HSV-2, but whose delivery cultures were negative, were positive by polymerase chain reaction and no false-positive reactions were obtained when the reaction mixture contained human cell DNA or varicella-zoster virus, cytomegalovirus, Epstein-Barr virus, or human papillomavirus DNA.

Original languageEnglish (US)
Pages (from-to)1031-1035
Number of pages5
JournalJournal of Infectious Diseases
Volume162
Issue number5
StatePublished - Nov 1990
Externally publishedYes

Fingerprint

Asymptomatic Infections
Simplexvirus
Human Herpesvirus 2
Pregnant Women
Human Herpesvirus 1
Polymerase Chain Reaction
DNA
False Positive Reactions
Herpes Genitalis
Human Herpesvirus 3
Ethidium
Lip
Cytomegalovirus
Human Herpesvirus 4
Gels
Staining and Labeling
Antibodies

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Use of polymerase chain reaction for successful identification of asymptomatic genital infection with herpes simplex virus in pregnant women at delivery. / Hardy, Deborah A.; Arvin, Ann M.; Yasukawa, Linda L.; Bronzan, Rachel N.; Lewinsohn, David; Hensleigh, Paul A.; Prober, Charles G.

In: Journal of Infectious Diseases, Vol. 162, No. 5, 11.1990, p. 1031-1035.

Research output: Contribution to journalArticle

Hardy, Deborah A. ; Arvin, Ann M. ; Yasukawa, Linda L. ; Bronzan, Rachel N. ; Lewinsohn, David ; Hensleigh, Paul A. ; Prober, Charles G. / Use of polymerase chain reaction for successful identification of asymptomatic genital infection with herpes simplex virus in pregnant women at delivery. In: Journal of Infectious Diseases. 1990 ; Vol. 162, No. 5. pp. 1031-1035.
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