Use of peptides to select for anti-rhodopsin antibodies with desired amino acid sequence specificities.

G. Adamus, A. Arendt, Z. S. Zam, J. H. McDowell, P. A. Hargrave

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

We have produced monoclonal antibodies directed against defined amino acid sequences of rhodopsin by using peptides for hybridoma selection, rather than for immunization. Mice were immunized with rhodopsin, and hybridoma culture supernatants were assayed by ELISA to detect anti-rhodopsin antibodies. These antibodies were further tested by competition ELISA using synthetic peptides from the rhodopsin sequence in order to select antibodies with the desired amino acid sequence specificity. Site-specific monoclonal antibodies selected in this way have affinities for the protein which are typically higher than that of antibodies which have been generated against peptide-carrier conjugates as antigens.

Original languageEnglish (US)
Pages (from-to)42-47
Number of pages6
JournalPeptide research
Volume1
Issue number1
StatePublished - 1988

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Fingerprint Dive into the research topics of 'Use of peptides to select for anti-rhodopsin antibodies with desired amino acid sequence specificities.'. Together they form a unique fingerprint.

Cite this