Use of herpes virus amplicon vectors to study brain disorders

Rachael L. Neve, Kim Neve, Eric J. Nestler, William A. Carlezon

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

There is an enormous initiative to establish the genetic basis for disorders of brain function. Unfortunately, genetic intervention is not accomplished easily in the nervous system. One strategy is to engineer and deliver to neurons specialized viral vectors that carry a gene (or genes) of interest, thereby exploiting the natural ability of viruses to insert genetic material into cells. When delivered to brain cells, these vectors cause infected cells to increase the expression of the genes of interest. The ability to deliver genes into neurons in vitro and in vivo with herpes simplex virus (HSV) amplicon vectors has made it possible to carry out exactly these sorts of experiments. This technology has the potential to offer new insights into the etiology of a wide variety of neuropsychiatric disorders. We describe the use of HSV amplicon vectors to study Alzheimer disease, drug addiction, and depression, and discuss the considerations that enter into the use of these vectors both in vitro and in vivo. The HSV amplicon virus is a user-friendly vector for the delivery of genes into neurons that has come of age for the study of brain function.

Original languageEnglish (US)
Pages (from-to)381-389
Number of pages9
JournalBioTechniques
Volume39
Issue number3
DOIs
StatePublished - 2005

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Brain Diseases
Viruses
Brain
Genes
Simplexvirus
Aptitude
Neurons
Inborn Genetic Diseases
Genetic Engineering
Die casting inserts
Nervous System
Neurology
Substance-Related Disorders
Alzheimer Disease
Depression
Technology
Gene Expression
Engineers
Pharmaceutical Preparations
Experiments

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Use of herpes virus amplicon vectors to study brain disorders. / Neve, Rachael L.; Neve, Kim; Nestler, Eric J.; Carlezon, William A.

In: BioTechniques, Vol. 39, No. 3, 2005, p. 381-389.

Research output: Contribution to journalArticle

Neve, RL, Neve, K, Nestler, EJ & Carlezon, WA 2005, 'Use of herpes virus amplicon vectors to study brain disorders', BioTechniques, vol. 39, no. 3, pp. 381-389. https://doi.org/10.2144/05393PS01
Neve, Rachael L. ; Neve, Kim ; Nestler, Eric J. ; Carlezon, William A. / Use of herpes virus amplicon vectors to study brain disorders. In: BioTechniques. 2005 ; Vol. 39, No. 3. pp. 381-389.
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