Use of controlled ovulation of the dominant follicle to assess oocyte maturation during natural menstrual cycles in rhesus macaques

Jeffrey T. Jensen; Jessica E. Stanley; Mary B. Zelinski; Richard L. Stouffer; Darla Jacob; John Fanton

doi:10.1016/j.fertnstert.2006.11.059

Use of controlled ovulation of the dominant follicle to assess oocyte maturation during natural menstrual cycles in rhesus macaques

Jeffrey T. Jensen, Jessica E. Stanley, Mary B. Zelinski, Richard L. Stouffer, Darla Jacob, John Fanton

Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

To determine the practicality of controlled ovulation of the dominant follicle as a technique to study meiotic maturation of oocytes during contraceptive research, we developed a technique for aspiration of the single dominant follicle using a dual-needle continuous irrigation technique 27 hours after an ovulatory stimulus. All of the oocytes (3/3) recovered from control animals, but only 1/6 (17%) of oocytes from animals treated with the meiotic inhibitor ORG 9935 exhibited germinal vesicle breakdown, indicating resumption of meiosis.

Original language	English (US)
Pages (from-to)	1477-1479
Number of pages	3
Journal	Fertility and sterility
Volume	87
Issue number	6
DOIs	https://doi.org/10.1016/j.fertnstert.2006.11.059
State	Published - Jun 2007

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

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10.1016/j.fertnstert.2006.11.059

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title = "Use of controlled ovulation of the dominant follicle to assess oocyte maturation during natural menstrual cycles in rhesus macaques",

abstract = "To determine the practicality of controlled ovulation of the dominant follicle as a technique to study meiotic maturation of oocytes during contraceptive research, we developed a technique for aspiration of the single dominant follicle using a dual-needle continuous irrigation technique 27 hours after an ovulatory stimulus. All of the oocytes (3/3) recovered from control animals, but only 1/6 (17%) of oocytes from animals treated with the meiotic inhibitor ORG 9935 exhibited germinal vesicle breakdown, indicating resumption of meiosis.",

author = "Jensen, {Jeffrey T.} and Stanley, {Jessica E.} and Zelinski, {Mary B.} and Stouffer, {Richard L.} and Darla Jacob and John Fanton",

note = "Funding Information: Supported by the National Institute of Child Health and Human Development through grants HD042710 (R01-042710); U54 HD18185; U54 HD 031398; and NCRR RR00163. ",

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T1 - Use of controlled ovulation of the dominant follicle to assess oocyte maturation during natural menstrual cycles in rhesus macaques

AU - Jensen, Jeffrey T.

AU - Stanley, Jessica E.

AU - Zelinski, Mary B.

AU - Stouffer, Richard L.

AU - Jacob, Darla

AU - Fanton, John

N1 - Funding Information: Supported by the National Institute of Child Health and Human Development through grants HD042710 (R01-042710); U54 HD18185; U54 HD 031398; and NCRR RR00163.

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AB - To determine the practicality of controlled ovulation of the dominant follicle as a technique to study meiotic maturation of oocytes during contraceptive research, we developed a technique for aspiration of the single dominant follicle using a dual-needle continuous irrigation technique 27 hours after an ovulatory stimulus. All of the oocytes (3/3) recovered from control animals, but only 1/6 (17%) of oocytes from animals treated with the meiotic inhibitor ORG 9935 exhibited germinal vesicle breakdown, indicating resumption of meiosis.

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Use of controlled ovulation of the dominant follicle to assess oocyte maturation during natural menstrual cycles in rhesus macaques

Abstract

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