Use of controlled ovulation of the dominant follicle to assess oocyte maturation during natural menstrual cycles in rhesus macaques

Jeffrey T. Jensen, Jessica E. Stanley, Mary B. Zelinski, Richard L. Stouffer, Darla Jacob, John Fanton

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

To determine the practicality of controlled ovulation of the dominant follicle as a technique to study meiotic maturation of oocytes during contraceptive research, we developed a technique for aspiration of the single dominant follicle using a dual-needle continuous irrigation technique 27 hours after an ovulatory stimulus. All of the oocytes (3/3) recovered from control animals, but only 1/6 (17%) of oocytes from animals treated with the meiotic inhibitor ORG 9935 exhibited germinal vesicle breakdown, indicating resumption of meiosis.

Original languageEnglish (US)
Pages (from-to)1477-1479
Number of pages3
JournalFertility and sterility
Volume87
Issue number6
DOIs
StatePublished - Jun 1 2007

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ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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