Use of assisted reproductive technologies in the propagation of rhesus macaque offspring

D. P. Wolf, S. Thormahlen, C. Ramsey, R. R. Yeoman, J. Fanton, Shoukhrat Mitalipov

    Research output: Contribution to journalArticle

    56 Citations (Scopus)

    Abstract

    The assisted reproductive technologies (ARTs) as tailored to the production of rhesus monkeys at the Oregon National Primate Research Center (ONPRC) are described. Efficient fertilization of mature oocytes recovered by aspiration from females subjected to follicular stimulation was achieved with fresh or frozen sperm by intracytoplasmic sperm injection (ICSI). Embryo development to the early cleavage stage occurred at high frequency. Cryopreserved embryos showed high postthaw survival and were also transferred in efforts to establish pregnancies. Three methods of transfer were evaluated, two involving embryo placement into the oviduct, laparoscopy and minilaparotomy, and a nonsurgical, transcervical approach that resulted in uterine deposition. Early cleaving embryos (Days 1-4) were transferred into the oviducts of synchronized recipients with optimal results and pregnancy rates of up to 36%. Pregnancy rates were similar when two fresh or frozen embryos were transferred (28-30%), although more than two embryos had to be thawed to compensate for embryo loss during freeze-thawing. Normal gestational lengths, birth weights, and growth curves were seen with ART-produced infants compared with infants produced by natural mating in the timed mated breeding (TMB) colony at the ONPRC. In 72 singleton pregnancies established following the transfer of ART-produced embryos, the live-birth rate, at 87.5%, was statistically identical to that for the TMB colony. Further development of the ARTs should result in increasing use of these techniques to augment conventional approaches to propagating monkeys, especially those of defined genotypes.

    Original languageEnglish (US)
    Pages (from-to)486-493
    Number of pages8
    JournalBiology of Reproduction
    Volume71
    Issue number2
    DOIs
    StatePublished - Aug 2004

    Fingerprint

    Assisted Reproductive Techniques
    Macaca mulatta
    Embryonic Structures
    Oviducts
    Pregnancy Rate
    Primates
    Breeding
    Embryo Loss
    Oocyte Retrieval
    Pregnancy
    Intracytoplasmic Sperm Injections
    Birth Rate
    Live Birth
    Research
    Fertilization
    Birth Weight
    Laparoscopy
    Laparotomy
    Embryonic Development
    Haplorhini

    Keywords

    • Assisted reproductive technology
    • Embryo
    • In vitro fertilization
    • Pregnancy

    ASJC Scopus subject areas

    • Cell Biology
    • Developmental Biology
    • Embryology

    Cite this

    Use of assisted reproductive technologies in the propagation of rhesus macaque offspring. / Wolf, D. P.; Thormahlen, S.; Ramsey, C.; Yeoman, R. R.; Fanton, J.; Mitalipov, Shoukhrat.

    In: Biology of Reproduction, Vol. 71, No. 2, 08.2004, p. 486-493.

    Research output: Contribution to journalArticle

    Wolf, D. P. ; Thormahlen, S. ; Ramsey, C. ; Yeoman, R. R. ; Fanton, J. ; Mitalipov, Shoukhrat. / Use of assisted reproductive technologies in the propagation of rhesus macaque offspring. In: Biology of Reproduction. 2004 ; Vol. 71, No. 2. pp. 486-493.
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