Use of a lacZ fusion to study the role of the spoO genes of bacillus subtilis in developmental regulation

Peter Zuber, Richard Losick

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

A mutation in any one of eight spoO genes of Bacillus subtilis blocks the process of spore formation at its earliest stage. To investigate how the products of the spoO genes may be involved in developmental gene expression, we fused the lacZ gene of E. coli to spoVG, a sporulation gene whose induction at the onset of sporulation is under spoO control. In cells of Spo+ bacteria containing a single copy of the gene fusion, conditions leading to the onset of sporulation resulted in the induction of β-galactosidase synthesis. This induction was moderately to severely impaired by mutations in any of seven spoO genes. Deletion and hybridization analysis demonstrated that this sporulation-induced enzyme synthesis was exclusively expressed from the two overlapping promoters, which comprise the spoVG transcription-initiation region, and that a small DNA segment (157 bp) containing the spoVG promoters was sufficient to cause spoO-dependent induction of the fused lacZ gene.

Original languageEnglish (US)
Pages (from-to)275-283
Number of pages9
JournalCell
Volume35
Issue number1
DOIs
StatePublished - 1983
Externally publishedYes

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Bacilli
Bacillus subtilis
Fusion reactions
Genes
Lac Operon
Galactosidases
Developmental Genes
Mutation
Gene Fusion
Spores
Escherichia coli
Bacteria
Gene Expression
Transcription
DNA
Gene expression
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Use of a lacZ fusion to study the role of the spoO genes of bacillus subtilis in developmental regulation. / Zuber, Peter; Losick, Richard.

In: Cell, Vol. 35, No. 1, 1983, p. 275-283.

Research output: Contribution to journalArticle

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