Urea protects from the proapoptotic effect of NaCl in renal medullary cells

Zheng Zhang, Tian Wei, David Cohen

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Hypertonic NaCl upregulated two sensitive and specific biochemical indices of apoptosis, caspase-3 activation and annexin V binding, in a time- and dose-dependent fashion in renal medullary mIMCD3 cells. Pretreatment with urea (200 mM for 30 min) protected from the proapoptotic effect of hypertonic stress (200 mosmol/kgH2O) in this model. The protective effect of urea was dose dependent and was effective even when applied a short time (≤1 h) following NaCl exposure; this protective effect was not observed in the nonrenal 3T3 cell line. In both mIMCD3 and 3T3 cells, urea failed to protect from the proapoptotic stressor, ultraviolet (UV)-B irradiation. The ability of urea to protect from hypertonic stress was approximately comparable to the protective effect of peptide mitogens epidermal growth factor and insulin-like growth factor (IGF), but it potentiated the IGF effect. Interestingly, the tyrosine kinase inhibitor, genistein, potentiated the proapoptotic effect of urea yet abrogated the proapoptotic effect of hypertonic stress. In aggregate, these data indicate that urea protects from the proapoptotic effect of hypertonic stress in a potentially cell type-specific and stimulus-specific fashion.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume279
Issue number2 48-2
StatePublished - 2000

Fingerprint

Urea
Osmotic Pressure
Kidney
3T3 Cells
Somatomedins
Genistein
Annexin A5
Mitogens
Epidermal Growth Factor
Caspase 3
Protein-Tyrosine Kinases
Apoptosis
Cell Line
Peptides

Keywords

  • Annexin
  • Apoptosis
  • Caspase
  • Hypertonicity
  • Osmotic
  • Stress

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Urea protects from the proapoptotic effect of NaCl in renal medullary cells. / Zhang, Zheng; Wei, Tian; Cohen, David.

In: American Journal of Physiology - Renal Physiology, Vol. 279, No. 2 48-2, 2000.

Research output: Contribution to journalArticle

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AB - Hypertonic NaCl upregulated two sensitive and specific biochemical indices of apoptosis, caspase-3 activation and annexin V binding, in a time- and dose-dependent fashion in renal medullary mIMCD3 cells. Pretreatment with urea (200 mM for 30 min) protected from the proapoptotic effect of hypertonic stress (200 mosmol/kgH2O) in this model. The protective effect of urea was dose dependent and was effective even when applied a short time (≤1 h) following NaCl exposure; this protective effect was not observed in the nonrenal 3T3 cell line. In both mIMCD3 and 3T3 cells, urea failed to protect from the proapoptotic stressor, ultraviolet (UV)-B irradiation. The ability of urea to protect from hypertonic stress was approximately comparable to the protective effect of peptide mitogens epidermal growth factor and insulin-like growth factor (IGF), but it potentiated the IGF effect. Interestingly, the tyrosine kinase inhibitor, genistein, potentiated the proapoptotic effect of urea yet abrogated the proapoptotic effect of hypertonic stress. In aggregate, these data indicate that urea protects from the proapoptotic effect of hypertonic stress in a potentially cell type-specific and stimulus-specific fashion.

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