Abstract
Hypertonic NaCl upregulated two sensitive and specific biochemical indices of apoptosis, caspase-3 activation and annexin V binding, in a time- and dose-dependent fashion in renal medullary mIMCD3 cells. Pretreatment with urea (200 mM for 30 min) protected from the proapoptotic effect of hypertonic stress (200 mosmol/kgH2O) in this model. The protective effect of urea was dose dependent and was effective even when applied a short time (≤1 h) following NaCl exposure; this protective effect was not observed in the nonrenal 3T3 cell line. In both mIMCD3 and 3T3 cells, urea failed to protect from the proapoptotic stressor, ultraviolet (UV)-B irradiation. The ability of urea to protect from hypertonic stress was approximately comparable to the protective effect of peptide mitogens epidermal growth factor and insulin-like growth factor (IGF), but it potentiated the IGF effect. Interestingly, the tyrosine kinase inhibitor, genistein, potentiated the proapoptotic effect of urea yet abrogated the proapoptotic effect of hypertonic stress. In aggregate, these data indicate that urea protects from the proapoptotic effect of hypertonic stress in a potentially cell type-specific and stimulus-specific fashion.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Renal Physiology |
| Volume | 279 |
| Issue number | 2 48-2 |
| State | Published - 2000 |
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Keywords
- Annexin
- Apoptosis
- Caspase
- Hypertonicity
- Osmotic
- Stress
ASJC Scopus subject areas
- Physiology
- Physiology (medical)
Cite this
Urea protects from the proapoptotic effect of NaCl in renal medullary cells. / Zhang, Zheng; Wei, Tian; Cohen, David.
In: American Journal of Physiology - Renal Physiology, Vol. 279, No. 2 48-2, 2000.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Urea protects from the proapoptotic effect of NaCl in renal medullary cells
AU - Zhang, Zheng
AU - Wei, Tian
AU - Cohen, David
PY - 2000
Y1 - 2000
N2 - Hypertonic NaCl upregulated two sensitive and specific biochemical indices of apoptosis, caspase-3 activation and annexin V binding, in a time- and dose-dependent fashion in renal medullary mIMCD3 cells. Pretreatment with urea (200 mM for 30 min) protected from the proapoptotic effect of hypertonic stress (200 mosmol/kgH2O) in this model. The protective effect of urea was dose dependent and was effective even when applied a short time (≤1 h) following NaCl exposure; this protective effect was not observed in the nonrenal 3T3 cell line. In both mIMCD3 and 3T3 cells, urea failed to protect from the proapoptotic stressor, ultraviolet (UV)-B irradiation. The ability of urea to protect from hypertonic stress was approximately comparable to the protective effect of peptide mitogens epidermal growth factor and insulin-like growth factor (IGF), but it potentiated the IGF effect. Interestingly, the tyrosine kinase inhibitor, genistein, potentiated the proapoptotic effect of urea yet abrogated the proapoptotic effect of hypertonic stress. In aggregate, these data indicate that urea protects from the proapoptotic effect of hypertonic stress in a potentially cell type-specific and stimulus-specific fashion.
AB - Hypertonic NaCl upregulated two sensitive and specific biochemical indices of apoptosis, caspase-3 activation and annexin V binding, in a time- and dose-dependent fashion in renal medullary mIMCD3 cells. Pretreatment with urea (200 mM for 30 min) protected from the proapoptotic effect of hypertonic stress (200 mosmol/kgH2O) in this model. The protective effect of urea was dose dependent and was effective even when applied a short time (≤1 h) following NaCl exposure; this protective effect was not observed in the nonrenal 3T3 cell line. In both mIMCD3 and 3T3 cells, urea failed to protect from the proapoptotic stressor, ultraviolet (UV)-B irradiation. The ability of urea to protect from hypertonic stress was approximately comparable to the protective effect of peptide mitogens epidermal growth factor and insulin-like growth factor (IGF), but it potentiated the IGF effect. Interestingly, the tyrosine kinase inhibitor, genistein, potentiated the proapoptotic effect of urea yet abrogated the proapoptotic effect of hypertonic stress. In aggregate, these data indicate that urea protects from the proapoptotic effect of hypertonic stress in a potentially cell type-specific and stimulus-specific fashion.
KW - Annexin
KW - Apoptosis
KW - Caspase
KW - Hypertonicity
KW - Osmotic
KW - Stress
UR - http://www.scopus.com/inward/record.url?scp=0033848559&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033848559&partnerID=8YFLogxK
M3 - Article
C2 - 10919855
AN - SCOPUS:0033848559
VL - 279
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 2 48-2
ER -