Urea activates ribosomal s6 kinase (RSK) in a MEK-dependent fashion in renal mIMCD3 cells

Zheng Zhang, David Cohen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Urea activates a characteristic subset of signaling pathways in a tissue-specific fashion, including transcription of immediate early genes through activation of the mitogen-activated protein kinase (MAPK), ERK (extracellular signal-regulated kinase), and activation of its transcription factor substrate, Elk-1. The ability of urea to activate the ERK effector and pivotal regulatory kinase, ribosomal S6 kinase (RSK), was investigated in mIMCD3 renal inner medullary collecting duct cells. Urea upregulated RSK activity in a time-dependent fashion in serum-deprived mIMCD3 cells; the effect was maximal at 5 min. Activation by hypertonic NaCl, in contrast, was negligible at 5 min and peaked at 15 min. Both stimuli induced the nuclear translocation of cytosolic RSK, as determined via immunofluorescence. Importantly, activation of RSK by both solutes was MAPK/ERK kinase (MEK) dependent, as determined by the ability of the specific MEK inhibitor, PD- 98059, to abrogate the response. Taken together, these data indicate that urea activates the ERK effector, RSK, in cells of the renal medulla in an ERK-dependent fashion, further emphasizing the functional significance of urea signaling through ERK activation in renal medullary cells.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume274
Issue number1 43-1
StatePublished - Jan 1998

Fingerprint

Ribosomal Protein S6 Kinases
Mitogen-Activated Protein Kinase Kinases
Extracellular Signal-Regulated MAP Kinases
Urea
Kidney
Mitogen-Activated Protein Kinases
ets-Domain Protein Elk-1
Phosphotransferases
Immediate-Early Genes
Transcriptional Activation
Fluorescent Antibody Technique
Serum

Keywords

  • Cell culture
  • Hypertonicity
  • Kidney
  • Renal medullary cells
  • Signal transduction

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Urea activates ribosomal s6 kinase (RSK) in a MEK-dependent fashion in renal mIMCD3 cells. / Zhang, Zheng; Cohen, David.

In: American Journal of Physiology - Renal Physiology, Vol. 274, No. 1 43-1, 01.1998.

Research output: Contribution to journalArticle

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