Uptake of fluorescent gentamicin by vertebrate sensory cells in vivo

C. F. Dai, D. Mangiardi, D. A. Cotanche, Peter Steyger

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

Aminoglycoside uptake in the inner ear remains poorly understood. We subcutaneously injected a fluorescently-conjugated aminoglycoside, gentamicin-Texas Red (GTTR), to investigate the in vivo uptake of GTTR in the inner ear of several vertebrates, and in various murine sensory cells using confocal microscopy. In bullfrogs, GTTR uptake was prominent in mature hair cells, but not in immature hair cells. Avian hair cells accrued GTTR more rapidly at the base of the basilar papilla. GTTR was associated with the hair bundle; and, in guinea pigs and mice, somatic GTTR fluorescence was initially diffuse before punctate (endosomal) fluorescence could be observed. A baso-apical gradient of intracellular GTTR uptake in guinea pig cochleae could only be detected at early time points (

Original languageEnglish (US)
Pages (from-to)64-78
Number of pages15
JournalHearing Research
Volume213
Issue number1-2
DOIs
Publication statusPublished - Mar 2006

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Keywords

  • Blood-labyrinth barrier
  • Gentamicin
  • Hair cells
  • Inner ear

ASJC Scopus subject areas

  • Sensory Systems

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